Ischiropoulos H, al-Mehdi A B
Institute for Environmental Medicine, University of Pennsylvania, School of Medicine, Philadelphia 19104, USA.
FEBS Lett. 1995 May 15;364(3):279-82. doi: 10.1016/0014-5793(95)00307-u.
Proteins are targets of reactive species and detection of oxidatively modified proteins is often used as an index of oxidative stress. Peroxynitrite is a strong oxidant formed by reaction of nitric oxide with superoxide. Using fatty acid-free bovine serum albumin as a model we examined peroxynitrite-mediated protein modifications. The reaction of protein with peroxynitrite resulted in the oxidation of tryptophan and cysteine, in the nitration of tyrosine, in the formation of dityrosine, in the production of 2,4 dinitrophenylhydrazine-reactive carbonyls and in protein fragmentation. The formation of 3-nitrotyrosine represents a specific peroxynitrite-mediated protein modification that is different from modifications mediated by reactive oxygen species.
蛋白质是活性物质的作用靶点，氧化修饰蛋白质的检测常被用作氧化应激的指标。过氧亚硝酸根是一氧化氮与超氧阴离子反应生成的一种强氧化剂。我们以无脂肪酸牛血清白蛋白为模型，研究了过氧亚硝酸根介导的蛋白质修饰。蛋白质与过氧亚硝酸根的反应导致色氨酸和半胱氨酸氧化、酪氨酸硝化、二酪氨酸形成、2,4-二硝基苯肼反应性羰基生成以及蛋白质片段化。3-硝基酪氨酸的形成代表了一种特定的过氧亚硝酸根介导的蛋白质修饰，它不同于活性氧介导的修饰。
