Huet J C, Pernollet J C
Département de Physiologie et Biochimie Végétales, INRA Versailles, France.
Phytochemistry. 1993 Jul;33(4):797-805. doi: 10.1016/0031-9422(93)85277-x.
Elicitins are toxic proteins secreted by Phytophthora spp. responsible for the incompatible reaction and systemic leaf necroses on tobacco. We found that Phytophthora megasperma megasperma produces two elicitin isoforms, each belonging to a different physico-chemical class, although the beta elicitin was less toxic than other beta ones. These 98 residue proteins were purified, sequenced and compared with other known elicitins. In addition to the point mutation already known to correlate with the differences in necrotic activities between alpha and beta isoforms, we found another region of the molecule likely to be involved in the regulation of the toxicity.
激发素是疫霉属分泌的毒性蛋白，可引发烟草的非亲和反应和系统性叶片坏死。我们发现，大雄疫霉产生两种激发素亚型，每种属于不同的物理化学类别，尽管β-激发素的毒性低于其他β-激发素。这些含有98个氨基酸残基的蛋白质经过纯化、测序，并与其他已知激发素进行了比较。除了已知的与α和β亚型坏死活性差异相关的点突变外，我们还发现分子的另一个区域可能参与毒性调节。
