Huet J C, Nespoulous C, Pernollet J C
Département de Physiologie et Biochimie végétales, INRA Versailles, France.
Phytochemistry. 1992 May;31(5):1471-6. doi: 10.1016/0031-9422(92)83089-h.
Most of the phytopathogenic fungi Phytophthora secrete holoproteins (elicitins) responsible for the incompatible reaction and systemic leaf necroses on tobacco. We found that Phytophthora drechsleri produces several elicitin isoforms of various toxicity on tobacco. The CD spectra showed that their secondary structure was largely conserved, exhibiting ca 50% alpha-helix and little or no beta-structure. These 98 residue proteins were sequenced and compared with other known elicitins. Only one point mutation correlated with the differences in necrotic activities. This residue could be either an active or a regulatory site, involved in the interaction with a receptor responsible for necrosis induction.
大多数疫霉属植物病原真菌会分泌负责烟草上不亲和反应和系统性叶片坏死的全蛋白（激发素）。我们发现寄生疫霉会产生几种对烟草具有不同毒性的激发素亚型。圆二色光谱表明它们的二级结构基本保守，约50%为α-螺旋，几乎没有β-结构。对这些98个氨基酸残基的蛋白质进行了测序，并与其他已知激发素进行了比较。只有一个点突变与坏死活性的差异相关。这个残基可能是一个活性位点或调节位点，参与与负责坏死诱导的受体的相互作用。
