Inhibition of DNAse activity in PFGE analysis of DNA from Campylobacter jejuni.
作者信息
Gibson J R, Sutherland K, Owen R J
机构信息
National Collection of Type Cultures, Central Public Health Laboratory, London, UK.
出版信息
Lett Appl Microbiol. 1994 Nov;19(5):357-8. doi: 10.1111/j.1472-765x.1994.tb00474.x.
Abstract
DNAse-positive strains of Campylobacter jejuni degrade their chromosomal DNA during standard preparative procedures before pulsed-field gel electrophoresis (PFGE). A simple method for inactivation of this DNAse activity is described. Formaldehyde fixation of the bacterial cells resulted in the preservation of the DNA in a state suitable for restriction digestion and subsequent electrophoretic analysis.
摘要
Zotero 插件