Brunsbach F R, Reineke W
Bergische Universität-Gesamthochschule Wuppertal, Chemische Mikrobiologie, Germany.
Appl Microbiol Biotechnol. 1994 Nov;42(2-3):415-20. doi: 10.1007/BF00902751.
The microbial degradation of monochloro-, 1,2-dichloro-, 1,4-dichloro-, and 1,2,4-trichlorobenzene in soil slurries was examined with single compounds as well as in mixtures. The indigenous soil populations brought about the degradation of monochlorobenzene when incubated at 27 degrees C in slurries with 29% (w/w) suspended solids. In contrast, the other chlorobenzenes persisted during an incubation period of 1 month. Supplementation with buffer, mineral salts and acetate did not significantly influence the degradation. However, inoculation with Pseudomonas aeruginosa strain RHO1, a monochloro- and 1,4-dichlorobenzene-degrading organism, to a titre of 1 x 10(5) cells/g soil, led to rapid and complete degradation of 0.8 mM growth substrate within 30 h. In addition, the strain was able to degrade 1,2-dichloro- and 1,2,4-trichlorobenzene with stoichiometric release of chloride in the presence of acetate, ethanol, monochloro- or 1,4-dichlorobenzene as growth substrates. In mixtures of chlorobenzenes the co-metabolism of 1,2-dichloro- and 1,2,4-trichlorobenzene occurred until the growth substrates monochloro- and 1,4-dichlorobenzene were degraded. The degradation was faster in the slurries of garden soil containing 8% organic carbon than in soil with the lower content of 2.6%.
研究了土壤泥浆中一氯苯、1,2 - 二氯苯、1,4 - 二氯苯和1,2,4 - 三氯苯的微生物降解情况，包括单一化合物以及混合物形式。当在含29%（w/w）悬浮固体的泥浆中于27℃培养时，土壤中的原生微生物群落实现了一氯苯的降解。相比之下，其他氯苯在1个月的培养期内持续存在。添加缓冲液、矿物盐和乙酸盐对降解没有显著影响。然而，接种铜绿假单胞菌菌株RHO1（一种能降解一氯苯和1,4 - 二氯苯的微生物），接种量为1×10⁵个细胞/克土壤，能在30小时内使0.8 mM的生长底物快速且完全降解。此外，在以乙酸盐、乙醇、一氯苯或1,4 - 二氯苯作为生长底物时，该菌株能够降解1,2 - 二氯苯和1,2,4 - 三氯苯，并按化学计量释放氯离子。在氯苯混合物中，直到生长底物一氯苯和1,4 - 二氯苯被降解之前，1,2 - 二氯苯和1,2,4 - 三氯苯会发生共代谢。与有机碳含量为2.6%的土壤泥浆相比，有机碳含量为8%的花园土壤泥浆中的降解速度更快。
