Wicher K, Wicher V, Wang M C
Int Arch Allergy Appl Immunol. 1976;51(3):284-97. doi: 10.1159/000231603.
Guinea pigs infected intradermally with Treponema pallidum Nichols strain were examined for the presence of lesions and for cellular and humoral response. Of the 26 guinea pigs (77%) demonstrated darkfield positive lesions between 6 and 20 days after infection. Animals divided into six groups of 4-5 in each were sacrificed between 2 and 24 weeks. The peritoneal exudate cells, obtained 4 days after injection of oil, were examined by the direct and the cells from lymphoid organs by the indirect capillary leukocyte migration method in the presence of 10 and 30 mug/ml of Reiter antigen. The peritoneal exudate cells responded with enhancement of leukocyte migration, reaching significant values between 6 and 24 weeks after injection. The cells from the lymphoid organs showed enhancement of leukocyte migration until the 4th week of infection. Treponemal antibodies, found in all animals, started to appear at the 4th week reaching a maximum titer at the 12th week after infection. The appearance of the antibodies correlated well (p less than 0.05) with the appearance of the enhancement of leukocyte migration. Wassermann antibodies were not detected throughout the 24 weeks of infection. The unique immune responses are discussed in view of the available data concerning natural acquired and experimental syphilis.
对皮内感染梅毒螺旋体Nichols株的豚鼠进行了病变检查以及细胞和体液反应检测。26只豚鼠（77%）在感染后6至20天出现暗视野阳性病变。将动物分成每组4 - 5只的六组，在2至24周期间处死。注射油4天后获得的腹腔渗出细胞，在存在10和30微克/毫升Reiter抗原的情况下，通过直接法进行检测，来自淋巴器官的细胞通过间接毛细管白细胞迁移法进行检测。腹腔渗出细胞表现为白细胞迁移增强，在注射后6至24周达到显著值。来自淋巴器官的细胞在感染第4周前表现出白细胞迁移增强。在所有动物中均发现梅毒螺旋体抗体，在感染后第4周开始出现，在第12周达到最高滴度。抗体的出现与白细胞迁移增强的出现相关性良好（p小于0.05）。在整个24周的感染过程中均未检测到瓦氏抗体。结合有关自然获得性和实验性梅毒的现有数据，对独特的免疫反应进行了讨论。
