Sartono E, Kruize Y C, Kurniawan A, van der Meide P H, Partono F, Maizels R M, Yazdanbakhsh M
Department of Parasitology, Leiden University, Netherlands.
J Infect Dis. 1995 Jun;171(6):1683-7. doi: 10.1093/infdis/171.6.1683.
Cellular immune responses to filarial antigens were examined in persons before and 1 year after beginning treatment with diethylcarbamazine (DEC). The subjects (17 microfilaremics, 13 asymptomatic amicrofilaremics, and 13 with elephantiasis) had not responded to Brgia malayi adult worm antigen (BmA) before chemotherapy. T cell proliferative responses to BmA improved significantly after therapy in the 3 clinical groups (P < .05) but was highest in the elephantiasis patients and asymptomatic amicrofilareimics. Cytokine release profiles after stimulation with parasite antigen were analyzed. Production of interferon (IFN)-gamma by BmA-stimulated mononuclear cells increased significantly after DEC treatment (geometric mean, 39.6-55.7 U/mL; P < .05), largely due to improved responses in elephantiasis patients and asymptomatic amicrofilaremics. In contrast, BmA-induced interleukin (IL)-4 release did not change significantly in these same patients after treatment. Thus, both microfilaremic and amicrofilaremic infections with B. malayi are associated with similar down-regulation of proliferative T cell function and IFN-gamma release.
在使用乙胺嗪（DEC）治疗前及治疗1年后，对人群针对丝虫抗原的细胞免疫反应进行了检测。研究对象包括17名微丝蚴血症患者、13名无症状无微丝蚴血症患者和13名患有象皮肿的患者，这些患者在化疗前对马来布鲁线虫成虫抗原（BmA）均无反应。在这3个临床组中，治疗后T细胞对BmA的增殖反应显著改善（P < 0.05），但在象皮肿患者和无症状无微丝蚴血症患者中反应最高。分析了用寄生虫抗原刺激后的细胞因子释放情况。DEC治疗后，BmA刺激的单核细胞产生的干扰素（IFN）-γ显著增加（几何平均数，39.6 - 55.7 U/mL；P < 0.05），这主要是由于象皮肿患者和无症状无微丝蚴血症患者反应改善。相比之下，治疗后这些患者中BmA诱导的白细胞介素（IL）-4释放没有显著变化。因此，马来布鲁线虫的微丝蚴血症感染和无微丝蚴血症感染均与增殖性T细胞功能和IFN-γ释放的类似下调有关。
