单核细胞衍生的培养树突状细胞易受人类免疫缺陷病毒感染，并在名义抗原呈递过程中将病毒传播给静止的T细胞。
Monocyte-derived cultured dendritic cells are susceptible to human immunodeficiency virus infection and transmit virus to resting T cells in the process of nominal antigen presentation.
作者信息
Tsunetsugu-Yokota Y, Akagawa K, Kimoto H, Suzuki K, Iwasaki M, Yasuda S, Häusser G, Hultgren C, Meyerhans A, Takemori T
机构信息
Department of Immunology, National Institute of Health and AIDS Research Center, Tokyo, Japan.
出版信息
J Virol. 1995 Jul;69(7):4544-7. doi: 10.1128/JVI.69.7.4544-4547.1995.
Abstract
The susceptibility of monocyte-derived cultured dendritic cells (DCs) to human immunodeficiency virus (HIV) infection and their role in viral transmission in the immune response were studied in detail. We observed that highly purified cultured DCs were infected with the T-tropic Lai strain of HIV type 1 (HIV-1Lai) via the CD4 receptor, and this was followed by formation of the complete provirus as detected by PCR. HIV mRNAs were transcribed at only low levels, and virus production was undectable; however, the addition of the purified protein derivative antigen of tuberculin and of autologous resting T cells to HIV-1Lai-infected DCs but not to HIV-1Lai-infected macrophages led to massive HIV transmission and production. These data suggest that the interaction of infected DCs with T cells during the normal immune response could play an important role in the activation and expansion of HIV.
摘要
详细研究了单核细胞衍生的培养树突状细胞（DCs）对人类免疫缺陷病毒（HIV）感染的易感性及其在免疫反应中病毒传播的作用。我们观察到，高度纯化的培养DCs通过CD4受体被1型HIV的T嗜性Lai株（HIV-1Lai）感染，随后通过PCR检测到形成了完整的前病毒。HIV mRNA仅在低水平转录，且未检测到病毒产生；然而，将结核菌素纯化蛋白衍生物抗原和自体静息T细胞添加到HIV-1Lai感染的DCs中，而不是添加到HIV-1Lai感染的巨噬细胞中，会导致大量HIV传播和产生。这些数据表明，在正常免疫反应期间，受感染的DCs与T细胞的相互作用可能在HIV的激活和扩增中起重要作用。
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Monocyte-derived cultured dendritic cells are susceptible to human immunodeficiency virus infection and transmit virus to resting T cells in the process of nominal antigen presentation.单核细胞衍生的培养树突状细胞易受人类免疫缺陷病毒感染，并在名义抗原呈递过程中将病毒传播给静止的T细胞。
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本文引用的文献
1
HIV infection is active and progressive in lymphoid tissue during the clinically latent stage of disease.在疾病的临床潜伏期，HIV感染在淋巴组织中活跃且呈进行性发展。
Nature. 1993 Mar 25;362(6418):355-8. doi: 10.1038/362355a0.
2
Direct detection of HIV-1 RNA in epidermal Langerhans cells of HIV-infected patients.在HIV感染患者的表皮朗格汉斯细胞中直接检测HIV-1 RNA
J Acquir Immune Defic Syndr (1988). 1993 Apr;6(4):329-33.
3
Mechanisms of immune activation of human immunodeficiency virus in monocytes/macrophages.人类免疫缺陷病毒在单核细胞/巨噬细胞中的免疫激活机制。
J Virol. 1993 Oct;67(10):5713-20. doi: 10.1128/JVI.67.10.5713-5720.1993.
4
Rapid dendritic cell recruitment is a hallmark of the acute inflammatory response at mucosal surfaces.快速的树突状细胞募集是黏膜表面急性炎症反应的一个标志。
J Exp Med. 1994 Apr 1;179(4):1331-6. doi: 10.1084/jem.179.4.1331.
5
Efficient presentation of soluble antigen by cultured human dendritic cells is maintained by granulocyte/macrophage colony-stimulating factor plus interleukin 4 and downregulated by tumor necrosis factor alpha.培养的人树突状细胞对可溶性抗原的有效呈递可通过粒细胞/巨噬细胞集落刺激因子加白细胞介素4得以维持，而被肿瘤坏死因子α下调。
J Exp Med. 1994 Apr 1;179(4):1109-18. doi: 10.1084/jem.179.4.1109.
6
Dendritic cells freshly isolated from human blood express CD4 and mature into typical immunostimulatory dendritic cells after culture in monocyte-conditioned medium.从人血液中新鲜分离的树突状细胞表达CD4，并在单核细胞条件培养基中培养后成熟为典型的免疫刺激性树突状细胞。
J Exp Med. 1993 Sep 1;178(3):1067-76. doi: 10.1084/jem.178.3.1067.
7
T-cell dysfunction in HIV infection: anergy due to defective antigen-presenting cell function?HIV感染中的T细胞功能障碍：抗原呈递细胞功能缺陷导致的无反应性？
Immunol Today. 1993 Apr;14(4):161-4. doi: 10.1016/0167-5699(93)90279-T.
8
Massive covert infection of helper T lymphocytes and macrophages by HIV during the incubation period of AIDS.在艾滋病潜伏期，人类免疫缺陷病毒对辅助性T淋巴细胞和巨噬细胞进行大量隐匿性感染。
Nature. 1993 Mar 25;362(6418):359-62. doi: 10.1038/362359a0.
9
Proliferating dendritic cell progenitors in human blood.人类血液中增殖的树突状细胞祖细胞。
J Exp Med. 1994 Jul 1;180(1):83-93. doi: 10.1084/jem.180.1.83.
10
Macrophage-tropic HIV: critical for AIDS pathogenesis?嗜巨噬细胞性HIV：对艾滋病发病机制至关重要？
Immunol Today. 1994 Jul;15(7):332-9. doi: 10.1016/0167-5699(94)90081-7.
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