Knudsen S, Saadbye P, Hansen L H, Collier A, Jacobsen B L, Schlundt J, Karlström O H
Center for Environmental Diagnostics and Bioremediation, University of West Florida 32561.
Appl Environ Microbiol. 1995 Mar;61(3):985-91. doi: 10.1128/aem.61.3.985-991.1995.
We have developed very efficient suicide functions for biological containment based on the lethal Escherichia coli relF gene. The suicide functions are placed in duplicate within a plasmid and arranged to prevent inactivation by deletion, recombination, and insertional inactivation. The efficiency of this concept was tested in a plasmid containment system that prevents transfer of plasmids to wild-type bacteria. Protection against plasmid transfer was assayed in test tubes and in rat intestine. Protection was efficient and refractory to inactivation by mutation and transposons. The efficiency of the suicide system was also tested in soil and seawater. We show that unprecedented suicide efficiency can be achieved in soil and seawater after suicide induction by IPTG (isopropyl-beta-D-thiogalactopyranoside). More than 7 orders of magnitude reduction in suicide bacteria was achieved.
我们基于致死性大肠杆菌relF基因开发了用于生物遏制的高效自杀功能。自杀功能以一式两份的形式置于质粒中，并进行了安排以防止因缺失、重组和插入失活而失活。这一概念的效率在一个防止质粒转移至野生型细菌的质粒遏制系统中进行了测试。在试管和大鼠肠道中测定了对质粒转移的防护作用。防护作用高效且对突变和转座子失活具有抗性。自杀系统的效率也在土壤和海水中进行了测试。我们表明，在用IPTG（异丙基-β-D-硫代半乳糖吡喃糖苷）诱导自杀后，在土壤和海水中可实现前所未有的自杀效率。自杀细菌数量减少了超过7个数量级。
