Hill A W, Shears A L, Hibbitt K G
Infect Immun. 1976 Jul;14(1):257-65. doi: 10.1128/iai.14.1.257-265.1976.
Small amounts of endotoxin injected intramuscularly into cows induced endotoxin pyrogenic tolerance and an increase in the rate at which the serum killed a strain of Escherichia coli. Most of the difference between normal serum and serum from the endotoxin-tolerant animal was shown to be due to a bentonite-adsorbable factor other than lysozyme or beta-lysin. The antibacterial activity was not completely removed from either type of serum after bentonite adsorption. Electron microscope studies and measurement of the rate of release of radioactively labeled cytoplasmic contents showed that the bentonite-adsorbable factor was important in the final breakdown of the cell membrane and release of cellular contents. The antibacterial system was totally dependent on complement, and the importance of antibodies could not be entirely ruled out because adsorption at O C with homologous cells eliminated the killing activity.
给奶牛肌肉注射少量内毒素可诱导内毒素热原耐受性，并提高血清杀灭大肠杆菌菌株的速率。结果表明，正常血清与内毒素耐受动物血清之间的大部分差异是由一种除溶菌酶或β-溶素之外的可被膨润土吸附的因子所致。膨润土吸附后，两种血清中的抗菌活性均未被完全去除。电子显微镜研究以及对放射性标记细胞质内容物释放速率的测量表明，可被膨润土吸附的因子在细胞膜的最终破裂和细胞内容物的释放中起重要作用。抗菌系统完全依赖补体，并且由于在0℃下用同源细胞吸附可消除杀伤活性，因此不能完全排除抗体的重要性。
