肺隐球菌病患者中新型隐球菌基因的检测
Detection of Cryptococcus neoformans gene in patients with pulmonary cryptococcosis.
作者信息
Tanaka K, Miyazaki T, Maesaki S, Mitsutake K, Kakeya H, Yamamoto Y, Yanagihara K, Hossain M A, Tashiro T, Kohno S
机构信息
Second Department of Internal Medicine, Nagasaki University School of Medicine, Japan.
出版信息
J Clin Microbiol. 1996 Nov;34(11):2826-8. doi: 10.1128/jcm.34.11.2826-2828.1996.
Abstract
Pulmonary cryptococcosis was diagnosed by nested PCR. Extraction of DNA was performed by mechanical destruction of the capsules of Cryptococcus neoformans by the glass bead technique. Nested PCR was positive for 4 of 5 culture-positive specimens but negative for 1 culture-positive specimen, 10 culture-negative specimens, and 1 specimen with undetermined culture results.
摘要
肺隐球菌病通过巢式聚合酶链反应（PCR）诊断。采用玻璃珠技术机械破坏新型隐球菌的荚膜来提取DNA。5份培养阳性标本中有4份巢式PCR呈阳性，但1份培养阳性标本、10份培养阴性标本以及1份培养结果未确定的标本巢式PCR呈阴性。
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