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Development of a singleplex PCR assay for rapid identification and differentiation of Cryptococcus neoformans var. grubii, Cryptococcus neoformans var. neoformans, Cryptococcus gattii, and hybrids.建立一种用于快速鉴定和区分新型隐球菌变种格特隐球菌、新型隐球菌、荚膜组织胞浆菌及其杂种的单重 PCR 检测方法。
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Random amplified polymorphic DNA analysis of clinically and environmentally isolated Cryptococcus neoformans in Nagasaki.长崎地区临床及环境分离新生隐球菌的随机扩增多态性DNA分析
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Hybridization probes for conventional DNA fingerprinting used as single primers in the polymerase chain reaction to distinguish strains of Cryptococcus neoformans.用于传统DNA指纹识别的杂交探针在聚合酶链反应中用作单引物，以区分新型隐球菌菌株。
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High detection rates of cryptococcal antigen in pulmonary cryptococcosis by Eiken latex agglutination test with pronase pretreatment.经链霉蛋白酶预处理的艾肯乳胶凝集试验对肺隐球菌病中隐球菌抗原的高检出率
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Detection of Bacteroides fragilis in clinical specimens by PCR.
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Restriction fragment length polymorphism analysis of Cryptococcus neoformans isolates from environmental (pigeon excreta) and clinical sources in New York City.对从纽约市环境（鸽粪）和临床来源分离出的新型隐球菌进行限制性片段长度多态性分析。
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The Eiken Latex test for detection of a cryptococcal antigen in cryptococcosis. Comparison with a monoclonal antibody-based latex agglutination test, Pastorex Cryptococcus.用于检测隐球菌病中隐球菌抗原的艾肯乳胶试验。与基于单克隆抗体的乳胶凝集试验（帕斯托雷克斯隐球菌试验）的比较。
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Role of the latex agglutination test for cryptococcal antigen in diagnosing disseminated infections with Trichosporon beigelii.乳胶凝集试验检测新型隐球菌抗原在诊断白吉利丝孢酵母播散性感染中的作用。
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肺隐球菌病患者中新型隐球菌基因的检测

Detection of Cryptococcus neoformans gene in patients with pulmonary cryptococcosis.

作者信息

Tanaka K, Miyazaki T, Maesaki S, Mitsutake K, Kakeya H, Yamamoto Y, Yanagihara K, Hossain M A, Tashiro T, Kohno S

机构信息

Second Department of Internal Medicine, Nagasaki University School of Medicine, Japan.

出版信息

J Clin Microbiol. 1996 Nov;34(11):2826-8. doi: 10.1128/jcm.34.11.2826-2828.1996.

DOI:10.1128/jcm.34.11.2826-2828.1996
PMID:8897191
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC229412/
Abstract

Pulmonary cryptococcosis was diagnosed by nested PCR. Extraction of DNA was performed by mechanical destruction of the capsules of Cryptococcus neoformans by the glass bead technique. Nested PCR was positive for 4 of 5 culture-positive specimens but negative for 1 culture-positive specimen, 10 culture-negative specimens, and 1 specimen with undetermined culture results.

摘要

肺隐球菌病通过巢式聚合酶链反应（PCR）诊断。采用玻璃珠技术机械破坏新型隐球菌的荚膜来提取DNA。5份培养阳性标本中有4份巢式PCR呈阳性，但1份培养阳性标本、10份培养阴性标本以及1份培养结果未确定的标本巢式PCR呈阴性。