Schön W, Ziegler C, Gärtner H, Kraft G
Experimentalphysik III, Universität Gesamthochschule Kassel, Germany.
Radiat Environ Biophys. 1994;33(3):233-41. doi: 10.1007/BF01212679.
Human erythrocytes were irradiated with heavy ions of energies between 4 and 18 MeV/u having linear energy transfer (LET) values between 92 and 14,000 keV/microns. Hemolysis has been studied as a macroscopic parameter for membrane damage and changes of the fluidity as a more microscopic parameter. The membrane fluidity changed in a characteristic dose-dependent manner as detected by electron spin resonance employing 12-doxylstearic acid methyl ester spin label (SL 12). Lysis cross sections and RBE values were determined from dose effect curves. The results demonstrate a high hemolytic efficiency of heavy ions compared to X rays. With increasing LET values the measured relative biological efficiency (RBE) values increase continuously. In the complete LET range the cross sections formed one common curve as function of LET and no saturation effects are observed. This is in direct contrast to other biological endpoints such as cell inactivation or DNA damage.
用能量在4至18MeV/u之间、线性能量传递（LET）值在92至14,000keV/微米之间的重离子辐照人类红细胞。溶血作为膜损伤的宏观参数以及作为更微观参数的流动性变化已被研究。通过使用12 - 二氧硬脂酸甲酯自旋标记（SL 12）的电子自旋共振检测到，膜流动性以特征性的剂量依赖性方式发生变化。从剂量效应曲线确定裂解截面和相对生物效应（RBE）值。结果表明，与X射线相比，重离子具有高溶血效率。随着LET值增加，测得的相对生物效应（RBE）值持续增加。在整个LET范围内，截面形成一条作为LET函数的共同曲线，未观察到饱和效应。这与其他生物学终点如细胞失活或DNA损伤形成直接对比。
