Nakagawa H, Mikawa Y, Watanabe R
Department of Orthopedic Surgery, Sabae National Hospital, Fukui, Japan.
Spine (Phila Pa 1976). 1994 Oct 1;19(19):2164-9. doi: 10.1097/00007632-199410000-00006.
A histologic and biochemical study of elastin in the posterior longitudinal ligament and spinal dura was performed using autopsy cadavers.
To provide a rationale for resiliency of the PLL and the spinal dura.
Among the spinal ligaments, the nuchal ligament and the ligamentum flavum have a very high elastin content, and some biochemical and morphologic studies of these ligaments have been published. No reports on the elastin content in the posterior longitudinal ligament or spinal dura have been published.
Specimens of the posterior longitudinal ligament and the dura collected from three cadavers were studied histologically. For quantitative analysis of elastin and collagen, the posterior longitudinal ligament specimens were collected from 22 cadavers and the dura specimens from 20 cadavers. Elastin was extracted using the hot alkali method of Lansing et al.
The elastic fibers in the posterior longitudinal ligament generally were arranged parallel in the cephalocaudal direction, whereas the fibers in the spinal dura had a more multidirectional arrangement. The elastin content of the posterior longitudinal ligament averaged 7.3% of the total dry weight and that of the spinal dura was 7.1% for the ventral aspect and 13.8% for the dorsal aspect. The elastin content of the spinal dura was about two times higher in all dorsal specimens than in the corresponding ventral specimens. The elastin content of the lower thoracic region was higher than that of any other region. The amino acid composition of the elastin in the posterior longitudinal ligament and spinal dura was similar to that previously determined in other spinal ligaments.
使用尸检尸体对后纵韧带和硬脊膜中的弹性蛋白进行组织学和生化研究。
为后纵韧带和硬脊膜的弹性提供理论依据。
在脊柱韧带中，项韧带和黄韧带的弹性蛋白含量非常高，并且已经发表了一些关于这些韧带的生化和形态学研究。尚未有关于后纵韧带或硬脊膜中弹性蛋白含量的报道。
对从三具尸体收集的后纵韧带和硬脊膜标本进行组织学研究。为了对弹性蛋白和胶原蛋白进行定量分析，从22具尸体收集后纵韧带标本，从20具尸体收集硬脊膜标本。使用兰辛等人的热碱法提取弹性蛋白。
后纵韧带中的弹性纤维通常沿头尾方向平行排列，而硬脊膜中的纤维排列方向更多。后纵韧带的弹性蛋白含量平均占总干重的7.3%，硬脊膜腹侧的弹性蛋白含量为7.1%，背侧为13.8%。所有背侧标本中硬脊膜的弹性蛋白含量约为相应腹侧标本的两倍。下胸部区域的弹性蛋白含量高于其他任何区域。后纵韧带和硬脊膜中弹性蛋白的氨基酸组成与先前在其他脊柱韧带中测定的相似。
