Sewell D L, Pfaller M A, Barry A L
Pathology and Laboratory Medicine Service, Veterans Affairs Medical Center, Portland, Oregon 97201.
J Clin Microbiol. 1994 Sep;32(9):2099-102. doi: 10.1128/jcm.32.9.2099-2102.1994.
A comparison of the E test, the broth microdilution test, and the reference broth macrodilution susceptibility test of the National Committee for Clinical Laboratory Standards for fluconazole susceptibility testing was performed with 238 clinical isolates of Candida species and Torulopsis (Candida) glabrata. An 80% inhibition endpoint MIC was determined by the reference broth macrodilution method after 48 h of incubation. The MICs obtained by the two study methods were read after 24 and 48 h of incubation. Overall, excellent agreement within 2 doubling dilutions was obtained between the broth microdilution and the broth macrodilution methods for the combined results for all species at both 24 h (93%) and 48 h (94%). The correlation of 24-h MIC endpoints between the E test and the broth macrodilution methods was 37% for T. glabrata, 56% for Candida tropicalis, 93% for Candida albicans, and 90% for other Candida species. The percent agreement at 48 h ranged from 34% for T. glabrata to 97% for Candida species other than C. albicans and C. tropicalis. These initial results support the further evaluation of the E test as an alternative method for fluconazole susceptibility testing of Candida species.
采用美国国家临床实验室标准委员会的氟康唑药敏试验的E试验、肉汤微量稀释试验和参考肉汤常量稀释药敏试验，对238株念珠菌属和光滑念珠菌（假丝酵母菌）临床分离株进行了比较。孵育48小时后，通过参考肉汤常量稀释法确定80%抑制终点的最低抑菌浓度（MIC）。两种研究方法获得的MIC在孵育24小时和48小时后读取。总体而言，对于所有菌种的合并结果，肉汤微量稀释法和肉汤常量稀释法在24小时（93%）和48小时（94%）时在2倍稀释范围内具有极佳的一致性。对于光滑念珠菌，E试验和肉汤常量稀释法在24小时时MIC终点的相关性为37%，热带念珠菌为56%，白色念珠菌为93%，其他念珠菌属为90%。48小时时的一致率范围为，光滑念珠菌为34%，白色念珠菌和热带念珠菌以外的念珠菌属为97%。这些初步结果支持进一步评估E试验作为念珠菌属氟康唑药敏试验替代方法的可行性。
