Matter L, Gorgievski-Hrisoho M, Germann D
Institute for Medical Microbiology, University of Bern, Switzerland.
J Clin Microbiol. 1994 Sep;32(9):2134-9. doi: 10.1128/jcm.32.9.2134-2139.1994.
We evaluated four tests for the detection of rubella virus-specific immunoglobulin M antibodies. Primarily, consecutive serum samples were tested by two different assays. Selected panels of sera from patients with proven or likely recent rubella and false-positive and true-negative results in the two primary assays were further tested with two recently developed, fully automated techniques. The four tests were comparable in overall accuracy, but their dynamic ranges may differ considerably. Ways to optimize the predictive values are discussed. We conclude that automated assays may be used without causing significant changes in diagnostic accuracy or distortions in notifications of the incidence of rubella compared with the use of established tools.
我们评估了四种检测风疹病毒特异性免疫球蛋白M抗体的试验。首先，采用两种不同的检测方法对连续的血清样本进行检测。从确诊或可能近期感染风疹的患者中选取的血清样本组以及在两种主要检测方法中出现假阳性和真阴性结果的样本，再用两种最新开发的全自动技术进行进一步检测。这四种检测方法在总体准确性上具有可比性，但它们的动态范围可能有很大差异。文中讨论了优化预测值的方法。我们得出结论，与使用现有工具相比，使用自动化检测方法不会导致诊断准确性出现显著变化，也不会在风疹发病率报告中造成扭曲。
