基于重组风疹病毒样颗粒的新型酶免疫测定法检测风疹病毒免疫球蛋白M抗体的评估
Evaluation of a new enzyme immunoassay based on recombinant Rubella virus-like particles for detection of immunoglobulin M antibodies to Rubella virus.
作者信息
Grangeot-Keros L, Enders G
机构信息
Department of Microbiology and Immunology, Hôpital Antoine Béclère, Clamart, France.
出版信息
J Clin Microbiol. 1997 Feb;35(2):398-401. doi: 10.1128/jcm.35.2.398-401.1997.
A new enzyme immunoassay for the detection of specific antibodies to rubella virus was evaluated at two different sites. This assay, the Roche Cobas Core Rubella IgM EIA recomb, uses a recombinant rubella virus-like particle and is based upon the immunoglobulin M (IgM) capture principle. It was compared to the Abbott IMx Rubella IgM test and to the Sorin ETI-RUBEK-M reverse test. The relative clinical specificities were 99.30% for the Roche test, 98.26% for the Abbott test, and 100% for the Sorin test. The relative clinical sensitivities were 100, 93.87, and 82.65%, respectively. In the case of most primary infections, IgM antibodies could be detected immediately at the onset of the disease and for up to 7 weeks. In the case of vaccinations, they could be detected between 3 and 12 weeks after vaccination.
在两个不同地点对一种用于检测风疹病毒特异性抗体的新型酶免疫测定法进行了评估。这种测定法，即罗氏 Cobas Core 风疹 IgM EIA 重组法，使用重组风疹病毒样颗粒，基于免疫球蛋白 M（IgM）捕获原理。将其与雅培 IMx 风疹 IgM 检测法和索林 ETI - RUBEK - M 反向检测法进行了比较。罗氏检测法的相对临床特异性为 99.30%，雅培检测法为 98.26%，索林检测法为 100%。相对临床敏感性分别为 100%、93.87%和 82.65%。在大多数原发性感染的情况下，疾病发作时即可立即检测到 IgM 抗体，且可持续长达 7 周。在接种疫苗的情况下，接种后 3 至 12 周可检测到 IgM 抗体。
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