Chavany C, Saison-Behmoaras T, Le Doan T, Puisieux F, Couvreur P, Hélène C
Laboratoire de Biophysique, Muséum National d'Histoire Naturelle, INSERM U 201, CNRS URA 481, Paris, France.
Pharm Res. 1994 Sep;11(9):1370-8. doi: 10.1023/a:1018923301967.
Oligonucleotides can be adsorbed on polyisohexylcyanoacrylate nanoparticles in the presence of hydrophobic quarternary ammonium salts. Oligonucleotides bound to nanoparticles are protected from nuclease attack both in buffer and in cell culture media. Cellular uptake of oligonucleotides is increased when they are adsorbed onto nanoparticles as a result of the capture of nanoparticles by an endocytic phagocytic pathway. Intracellular stability towards nucleolytic degradation is increased in the presence of nanoparticles. These results show that nanoparticles can be considered as convenient carriers for the protection and delivery of oligonucleotides to cells.
在疏水性季铵盐存在的情况下，寡核苷酸可吸附在聚异己基氰基丙烯酸酯纳米颗粒上。与纳米颗粒结合的寡核苷酸在缓冲液和细胞培养基中均受到保护，免受核酸酶攻击。当寡核苷酸吸附到纳米颗粒上时，由于内吞吞噬途径捕获纳米颗粒，寡核苷酸的细胞摄取量会增加。在纳米颗粒存在的情况下，对核酸降解的细胞内稳定性会提高。这些结果表明，纳米颗粒可被视为保护寡核苷酸并将其递送至细胞的便捷载体。
