Morris J A, Wray C, Sojka W J
Br J Exp Pathol. 1976 Jun;57(3):354-60.
Immunosuppressive agents were used to determine the relative importance of T and B lymphocytes in conferring protection to mice vaccinated with a live gal E mutant of Salmonella typhimurium, strain G30D. Lymphocyte transformation and serum agglutination tests showed that while cyclophosphamide (CPA) suppressed B lymphocytes, antilymphocyte sea (ALS) suppressed both T and B cells. The humoral response of vaccinated animals treated with ALS was therefore supplemented by the i.v. injection of serum from untreated vaccinated mice. CPA-treated mice could not control multiplication of the vaccinal strain which eventually killed them. There was little multiplication of the vaccinal strain in the controls and ALS-treated mice, all of which survived to challenge. The vaccinated controls and vaccinated ALS treated groups each survived infection with the challenge strain which was gradually eliminated. It was concluded that humoral immunity was of greater importance than cellular immunity in mice vaccinated i.p. with strain G30D.
使用免疫抑制剂来确定T淋巴细胞和B淋巴细胞在赋予接种鼠伤寒沙门氏菌活gal E突变株G30D的小鼠保护性方面的相对重要性。淋巴细胞转化试验和血清凝集试验表明，虽然环磷酰胺（CPA）抑制B淋巴细胞，但抗淋巴细胞血清（ALS）同时抑制T细胞和B细胞。因此，对于用ALS处理的接种动物，通过静脉注射未处理的接种小鼠的血清来补充其体液反应。经CPA处理的小鼠无法控制疫苗株的增殖，最终导致死亡。在对照组和经ALS处理的小鼠中，疫苗株几乎没有增殖，所有这些小鼠在受到攻击后都存活下来。接种的对照组和接种后经ALS处理的组均在感染攻击株后存活下来，攻击株逐渐被清除。得出的结论是，对于经腹腔接种G30D株的小鼠，体液免疫比细胞免疫更为重要。
