来自肠上皮的分离刷状缘的收缩。
Contraction of isolated brush borders from the intestinal epithelium.
作者信息
Rodewald R, Newman S B, Karnovsky M J
出版信息
J Cell Biol. 1976 Sep;70(3):541-54. doi: 10.1083/jcb.70.3.541.
Abstract
Brush borders isolated from epithelial cells from the small intestine of neonatal rats are able to contract in the presence of ATP and Mg2+; Ca2+ is not required. Contraction is characterized by a pinching-in of the plasma membrane in the region of the zonula adherens and a subsequent rounding of the brush borders. No movement or consistent shortening of the microvilli is observed. The contraction appears to involve the 5- to 7-nm diameter microfilaments in the terminal web which associate with the zonula adherens. These filaments bind heavy meromyosin as do the actin core filaments of the microvilli. A model for contraction is presented in which, in the intact cell, terminal web filaments and core filaments interact to produce shortening of the microvilli.
摘要
从新生大鼠小肠上皮细胞分离出的刷状缘，在存在ATP和Mg2+的情况下能够收缩；不需要Ca2+。收缩的特征是黏着小带区域的质膜向内凹陷，随后刷状缘变圆。未观察到微绒毛有移动或持续缩短的情况。收缩似乎涉及终末网中直径为5至7纳米的微丝，这些微丝与黏着小带相关联。这些微丝与微绒毛的肌动蛋白核心丝一样能结合重酶解肌球蛋白。本文提出了一个收缩模型，即在完整细胞中，终末网丝和核心丝相互作用导致微绒毛缩短。
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本文引用的文献
1
Electron microscope studies of the structure of the microvilli on principal epithelial cells of rat jejunum after treatment in hypo- and hypertonic saline.对大鼠空肠主要上皮细胞微绒毛结构在低渗和高渗盐溶液处理后的电子显微镜研究。
J Cell Biol. 1962 Jul;14(1):125-39. doi: 10.1083/jcb.14.1.125.
2
Studies on the structure of myosin.肌球蛋白结构研究。
J Mol Biol. 1962 Apr;4:293-308. doi: 10.1016/s0022-2836(62)80007-2.
3
FINE STRUCTURE OF CELL SURFACE SPECIALIZATIONS IN THE MATURING DUODENAL MUCOSA OF THE CHICK.雏鸡成熟十二指肠黏膜细胞表面特化结构的精细结构
J Cell Biol. 1964 Apr;21(1):75-85. doi: 10.1083/jcb.21.1.75.
4
5
The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.在电子显微镜检查中，将高pH值的柠檬酸铅用作电子不透明染色剂。
J Cell Biol. 1963 Apr;17(1):208-12. doi: 10.1083/jcb.17.1.208.
6
Junctional complexes in various epithelia.各种上皮组织中的连接复合体。
J Cell Biol. 1963 May;17(2):375-412. doi: 10.1083/jcb.17.2.375.
7
An electron microscopic study of the intestinal villus. I. The fasting animal.肠绒毛的电子显微镜研究。I. 禁食动物。
J Biophys Biochem Cytol. 1959 May 25;5(3):363-72. doi: 10.1083/jcb.5.3.363.
8
Staining of tissue sections for electron microscopy with heavy metals.用重金属对组织切片进行电子显微镜染色。
J Biophys Biochem Cytol. 1958 Jul 25;4(4):475-8. doi: 10.1083/jcb.4.4.475.
9
Filaments of Amoeba proteus. II. Binding of heavy meromyosin by thin filaments in motile cytoplasmic extracts.变形虫的细丝。II. 运动性细胞质提取物中细丝与重酶解肌球蛋白的结合。
J Cell Biol. 1971 Jan;48(1):216-9. doi: 10.1083/jcb.48.1.216.
10
Microfilaments in cellular and developmental processes.细胞与发育过程中的微丝。
Science. 1971 Jan 15;171(3967):135-43. doi: 10.1126/science.171.3967.135.
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