Blow D M, Chayen N E, Lloyd L F, Saridakis E
Blackett Laboratory, Imperial College, London, United Kingdom.
Protein Sci. 1994 Oct;3(10):1638-43. doi: 10.1002/pro.5560031003.
Control of nucleation may be needed to obtain a reliable supply of large protein crystals, when standard techniques give many small or twinned crystals. Heterogeneous nucleation may be controlled by the use of fine filters, with the elimination of airborne contaminants by working under paraffin oil. The area of contact with the supporting vessel also has an important effect. A heterogenous nucleant for lysozyme (identified earlier) has been shown to be effective for carboxypeptidase G2. Control of homogeneous nucleation (previously demonstrated by dilutions of a nucleating sample after various times of incubation) may also be achieved by incubating a sample at 1 temperature, where nucleation can occur, and changing the temperature to conditions where there is growth but no nucleation.
当标准技术产生许多小晶体或孪晶时，可能需要控制成核以获得可靠的大蛋白质晶体供应。可以通过使用精细过滤器来控制非均相成核，在石蜡油下工作以消除空气中的污染物。与支撑容器的接触面积也有重要影响。一种用于溶菌酶的非均相成核剂（先前已鉴定）已被证明对羧肽酶G2有效。控制均相成核（先前通过在不同孵育时间后稀释成核样品来证明）也可以通过在一个能发生成核的温度下孵育样品，然后将温度改变至只生长不成核的条件来实现。
