Nanda S K, Panda S K, Jameel S, Dasarathy S, Acharya S K
Department of Pathology and Gastroenterology, All India Institute of Medical Sciences, New Delhi.
Trop Gastroenterol. 1994 Jul-Sep;15(3):145-51.
Hepatitis C virus (HCV) ribonucleic acid (RNA) was tested for in a group of 16 defined non-B chronic hepatitis patients using specific reverse transcription polymerase chain reaction (RT-PCR). These were chosen from amongst 56 biopsy proven cases of chronic hepatitis of which majority (40) were positive for hepatitis B virus infection. Hepatitis C virus RNA could be demonstrated in 12 (75%) of remaining 16 cases. These include all seven patients positive for antibody to HCV. Two of these patients had past history of blood transfusion and in another two the clinical course started with severe acute liver disease. This study establishes the association of HCV with severe liver disease. The clinical and biochemical profiles are also discussed. In view of limited sensitivity of the antibody assays it is justified to develop diagnostic testes based on local strains.
采用特异性逆转录聚合酶链反应（RT-PCR）对一组16例确诊的非乙型慢性肝炎患者检测丙型肝炎病毒（HCV）核糖核酸（RNA）。这些患者选自56例经活检证实的慢性肝炎病例，其中大多数（40例）乙型肝炎病毒感染呈阳性。在其余16例患者中的12例（75%）检测到丙型肝炎病毒RNA。这包括所有7例抗HCV抗体呈阳性的患者。其中2例患者有输血史，另外2例患者的临床病程始于严重的急性肝病。本研究证实了HCV与严重肝病之间的关联。还讨论了临床和生化特征。鉴于抗体检测的敏感性有限，开发基于本地毒株的诊断检测方法是合理的。
