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一种从化脓性链球菌上清液中纯化化脓性链球菌热原性外毒素A的新方法。

A new procedure for the purification of streptococcal pyrogenic exotoxin A from Streptococcus pyogenes supernatant.

作者信息

Mascini E M, Hazenberg M A, Verhage L A, Holm S E, Verhoef J, van Dijk H

机构信息

Eijkman-Winkler Institute for Microbiology, Utrecht University Hospital, The Netherlands.

出版信息

Clin Diagn Lab Immunol. 1996 Nov;3(6):779-81. doi: 10.1128/cdli.3.6.779-781.1996.

DOI:10.1128/cdli.3.6.779-781.1996
PMID:8914775
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC170447/
Abstract

An important role in the pathogenesis of invasive group A streptococcal disease has been ascribed to the production of streptococcal pyrogenic exotoxin A. We present a new technique for the purification of streptococcal pyrogenic exotoxin A from Streptococcus pyogenes NY-5 supernate, which is highly efficient with respect to yield (35%), purity (> or = 99%), and time.

摘要

A群链球菌侵袭性疾病发病机制中一个重要作用已归因于化脓性链球菌致热外毒素A的产生。我们提出了一种从化脓性链球菌NY - 5培养上清液中纯化化脓性链球菌致热外毒素A的新技术，该技术在产量（35%）、纯度（≥99%）和时间方面效率很高。

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本文引用的文献

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Geographic and temporal distribution and molecular characterization of two highly pathogenic clones of Streptococcus pyogenes expressing allelic variants of pyrogenic exotoxin A (Scarlet fever toxin).表达致热外毒素A等位变体（猩红热毒素）的化脓性链球菌两个高致病性克隆的地理和时间分布及分子特征
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A purified group A streptococcal pyrogenic exotoxin. Physiochemical and biological properties including the enhancement of susceptibility to endotoxin lethal shock.一种纯化的A组链球菌致热外毒素。其理化和生物学特性，包括对内毒素致死性休克易感性的增强。
J Exp Med. 1970 Mar 1;131(3):611-22. doi: 10.1084/jem.131.3.611.
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Nucleotide sequence of the type A streptococcal exotoxin (erythrogenic toxin) gene from Streptococcus pyogenes bacteriophage T12.化脓性链球菌噬菌体T12的A型链球菌外毒素（致热外毒素）基因的核苷酸序列。
Infect Immun. 1986 Apr;52(1):144-50. doi: 10.1128/iai.52.1.144-150.1986.
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N Engl J Med. 1989 Jul 6;321(1):1-7. doi: 10.1056/NEJM198907063210101.
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