少关节型幼年类风湿关节炎中针对假定癌蛋白DEK的一种新型自身抗体。

A novel autoantibody to the putative oncoprotein DEK in pauciarticular onset juvenile rheumatoid arthritis.

作者信息

Szer I S, Sierakowska H, Szer W

机构信息

Division of Rheumatology, Children's Hospital Los Angeles, CA.

出版信息

J Rheumatol. 1994 Nov;21(11):2136-42.

PMID:7869324

Abstract

OBJECTIVE

To define the frequency of a novel autoantibody reactive with a 45 kDa protein in children with juvenile rheumatoid arthritis (JRA). This protein is expressed by the putative oncogene DEK associated with a subtype of acute myeloid leukemia.

METHODS

The sera of 158 children with JRA were analyzed for the presence of anti-DEK by immunoblotting using purified DEK protein and compared with sera of 109 children with other rheumatic diseases and 25 healthy controls with no connective tissue disease.

RESULTS

Antibodies to DEK were found significantly more frequently among children with JRA than among children with other rheumatic diseases or controls (p < 0.001). Among children with JRA, anti-DEK was significantly more often associated with pauciarticular onset than with poly-articular and systemic onset subtypes (77 vs 29 and 8%, respectively, p < 0.001). Anti-DEK was no more frequent among children with pauciarticular JRA complicated by iritis than among those without iritis (88 vs 71%, respectively). The frequency of anti-DEK in other rheumatic diseases varied from 0 in children with spondyloarthritis to 31% in scleroderma.

CONCLUSION

Antibodies to DEK are highly associated with pauciarticular onset JRA.

摘要

目的

确定幼年类风湿关节炎（JRA）患儿中与一种45 kDa蛋白反应的新型自身抗体的频率。该蛋白由与急性髓系白血病一种亚型相关的推定癌基因DEK表达。

方法

使用纯化的DEK蛋白通过免疫印迹分析158例JRA患儿血清中抗DEK的存在情况，并与109例其他风湿性疾病患儿的血清以及25例无结缔组织病的健康对照者的血清进行比较。

结果

JRA患儿中抗DEK抗体的发现频率显著高于其他风湿性疾病患儿或对照者（p < 0.001）。在JRA患儿中，抗DEK与少关节起病的相关性显著高于多关节起病和全身起病亚型（分别为77%、29%和8%，p < 0.001）。合并虹膜炎的少关节型JRA患儿中抗DEK的频率并不高于无虹膜炎的患儿（分别为88%和71%）。抗DEK在其他风湿性疾病中的频率从脊柱关节炎患儿中的0%到硬皮病患儿中的31%不等。