Radák Z, Lee K, Choi W, Sunoo S, Kizaki T, Oh-ishi S, Suzuki K, Taniguchi N, Ohno H, Asano K
Laboratory for Exercise Physiology, University of Tsukuba, Ibaraki, Japan.
Eur J Appl Physiol Occup Physiol. 1994;69(5):392-5. doi: 10.1007/BF00865401.
The effects were examined of 6-month intermittent hypobaric (4000 m) exposure on the antioxidant enzyme systems in soleus and tibialis muscles of rats. At the end of the 6-month experimental exposure, the six rats in both the exposed group and the control group were sacrificed. Immunoreactive mitochondrial superoxide dismutase (Mn-SOD) contents were measured as well as the activities of antioxidant enzymes [Mn-SOD, cytosolic SOD (Cu,Zn-SOD), catalase (CAT), and glutathione peroxidase (GPX)]. Thiobarbituric acid-reactive substances (TBARS) were also determined as an indicator of lipid peroxidation. The high altitude exposure resulted in a marked increase in TBARS content in soleus muscle, suggesting increased levels of oxygen free radicals. Conversely, significant decreases in both Mn-SOD content and activity in soleus muscle were noted after exposure. Such trends were not noticed in tibialis muscle. On the other hand, no significant changes in Cu,Zn-SOD, CAT, or GPX were observed in either muscle. These results suggested that the increases in lipid peroxidation were most probably a result of decreased Mn-SOD function which was more depressed in oxidative than in glycolytic muscle.
研究了6个月间歇性低压（4000米）暴露对大鼠比目鱼肌和胫骨前肌抗氧化酶系统的影响。在6个月的实验暴露结束时，处死暴露组和对照组的6只大鼠。测量免疫反应性线粒体超氧化物歧化酶（Mn-SOD）含量以及抗氧化酶[Mn-SOD、胞质SOD（Cu,Zn-SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPX）]的活性。还测定了硫代巴比妥酸反应性物质（TBARS）作为脂质过氧化的指标。高原暴露导致比目鱼肌中TBARS含量显著增加，表明氧自由基水平升高。相反，暴露后比目鱼肌中Mn-SOD含量和活性均显著降低。在胫骨前肌中未观察到此类趋势。另一方面，在两种肌肉中均未观察到Cu,Zn-SOD、CAT或GPX有显著变化。这些结果表明，脂质过氧化的增加很可能是由于Mn-SOD功能下降所致，在氧化型肌肉中比在糖酵解型肌肉中下降更明显。
