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相似文献

1
RNA chain elongation on a chromatin template.染色质模板上的RNA链延伸。
Nucleic Acids Res. 1976 Sep;3(9):2223-31. doi: 10.1093/nar/3.9.2223.
2
Transcription of brain chromatin by ribonucleic acid polymerases from brain nuclei and from Escherichia coli.用来自脑细胞核和大肠杆菌的核糖核酸聚合酶对脑染色质进行转录。
Biochem J. 1972 Dec;130(4):1095-9. doi: 10.1042/bj1301095.
3
Effect of estrogen on gene expression in the chick oviduct. V. Changes in the number of RNA polymerase binding and initiation sites in chromatin.雌激素对雏鸡输卵管基因表达的影响。V. 染色质中RNA聚合酶结合位点及起始位点数量的变化。
J Biol Chem. 1975 Jul 10;250(13):5175-82.
4
Effect of estrogen on gene expression in the chick oviduct. Studies on the initiation of RNA synthesis on chromatin in vitro.雌激素对鸡输卵管基因表达的影响。体外染色质上RNA合成起始的研究。
J Biol Chem. 1976 Sep 25;251(18):5565-74.
5
Release of template restriction in chromatin by nuclear 4.5s RNA.
Biochim Biophys Acta. 1977 Apr 4;475(3):501-13. doi: 10.1016/0005-2787(77)90065-x.
6
Deposition of histone H1 onto reconstituted nucleosome arrays inhibits both initiation and elongation of transcripts by T7 RNA polymerase.组蛋白H1沉积到重组核小体阵列上会抑制T7 RNA聚合酶转录的起始和延伸。
Nucleic Acids Res. 1995 Mar 25;23(6):1075-82. doi: 10.1093/nar/23.6.1075.
7
Control of RNA synthesis by chromatin proteins.染色质蛋白对RNA合成的调控。
Nucleic Acids Res. 1976 Jul;3(7):1659-70. doi: 10.1093/nar/3.7.1659.
8
The kinetics of E. coli RNA polymerase.大肠杆菌RNA聚合酶的动力学
Nucleic Acids Res. 1976 Sep;3(9):2207-22. doi: 10.1093/nar/3.9.2207.
9
Chick-erythrocyte chromatin as a template for RNA synthesis in vitro.鸡红细胞染色质作为体外RNA合成的模板。
Eur J Biochem. 1973 Dec 17;40(2):591-8. doi: 10.1111/j.1432-1033.1973.tb03231.x.
10
Accessibility of chromatin to DNA polymerase I and location of the F1 histone.染色质对DNA聚合酶I的可及性以及F1组蛋白的定位
Nucleic Acids Res. 1975 Jan;2(1):113-9. doi: 10.1093/nar/2.1.113.

引用本文的文献

1
Salt-induced structural changes in nucleosomes.盐诱导核小体的结构变化。
Nucleic Acids Res. 1977 Apr;4(4):853-66. doi: 10.1093/nar/4.4.853.
2
Androgenic regulation of elongation of polyribonucleotide chains on rat ventral-prostate chromatin.雄激素对大鼠腹侧前列腺染色质上多核糖核苷酸链延伸的调控。
Biochem J. 1978 Feb 15;170(2):211-8. doi: 10.1042/bj1700211.
3
Initiation and elongation of polyribonucleotide chains on rat ventral-prostate chromatin transcribed by homologous ribonucleic acid polymerase B.同源核糖核酸聚合酶B转录的大鼠腹侧前列腺染色质上多核糖核苷酸链的起始与延伸
Biochem J. 1977 Aug 15;166(2):189-98. doi: 10.1042/bj1660189.
4
Binding of E. coli RNA polymerase to chromatin subunits.大肠杆菌RNA聚合酶与染色质亚基的结合。
Nucleic Acids Res. 1978 Mar;5(3):925-32. doi: 10.1093/nar/5.3.925.
5
The sv40 transcription complex. I. Effect of viral chromatin proteins on endogenous RNA polymerase activity.猿猴空泡病毒40转录复合体。I.病毒染色质蛋白对内源性RNA聚合酶活性的影响。
Nucleic Acids Res. 1977 Dec;4(12):4261-77. doi: 10.1093/nar/4.12.4261.

本文引用的文献

1
The Biology of Isolated Chromatin: Chromosomes, biologically active in the test tube, provide a powerful tool for the study of gene action.孤立染色质的生物学：在试管中具有生物活性的染色体为研究基因作用提供了一个有力的工具。
Science. 1968 Jan 5;159(3810):47-56. doi: 10.1126/science.159.3810.47.
2
The kinetics of enzyme-catalyzed reactions with two or more substrates or products. III. Prediction of initial velocity and inhibition patterns by inspection.双底物或多底物及多产物酶催化反应的动力学。III. 通过观察预测初速度和抑制模式
Biochim Biophys Acta. 1963 Feb 12;67:188-96. doi: 10.1016/0006-3002(63)91816-x.
3
Dependence of the melting temperature of DNA on salt concentration.DNA熔解温度对盐浓度的依赖性。
Biopolymers. 1965;3(2):195-208. doi: 10.1002/bip.360030207.
4
Kinetics of the in vitro inhibition of transcription by actinomycin.放线菌素对转录的体外抑制动力学
J Mol Biol. 1970 Jun 14;50(2):421-38. doi: 10.1016/0022-2836(70)90202-0.
5
Template activity of partially dehistonized nucleohistones for DNA dependent RNA polymerase.
J Biochem. 1970 May;67(5):661-5. doi: 10.1093/oxfordjournals.jbchem.a129294.
6
Mechanism of inhibitory action of histones on DNA template activity in vitro.组蛋白在体外对DNA模板活性的抑制作用机制。
Nature. 1970 Oct 17;228(5268):245-7. doi: 10.1038/228245a0.
7
Transcription of the early region of bacteriophage T7: selective initiation with dinucleotides.噬菌体T7早期区域的转录：二核苷酸的选择性起始
J Mol Biol. 1973 Jun 25;77(2):255-77. doi: 10.1016/0022-2836(73)90335-5.
8
Transcription of DNA and chromatin with calf thymus RNA polymerase B in vitro.体外使用小牛胸腺RNA聚合酶B进行DNA和染色质的转录。
J Mol Biol. 1975 Jun 25;95(2):257-69. doi: 10.1016/0022-2836(75)90394-0.
9
The structure of the globin genes in chromatin.染色质中珠蛋白基因的结构。
Biochemistry. 1975 Jun 3;14(11):2489-95. doi: 10.1021/bi00682a031.

染色质模板上的RNA链延伸。

RNA chain elongation on a chromatin template.

作者信息

Solage A, Cedar H

出版信息

Nucleic Acids Res. 1976 Sep;3(9):2223-31. doi: 10.1093/nar/3.9.2223.

DOI:10.1093/nar/3.9.2223
PMID:787933
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC343078/
Abstract

The rate of RNA chain elongation has been measured with DNA and chromatin as template. RNA propagation on chromatin is about 50% of the rate found with DNA. Kinetic experiments demonstrate that the inhibition is not due to interference with the addition of the nucleoside triphosphates. Analysis of the dependence of propagation on the Tm of DNA shows that the chromatin proteins interfere with the translocation of the RNA polymerase along the DNA template.

摘要

已以DNA和染色质为模板测量了RNA链的延伸速率。RNA在染色质上的延伸速率约为在DNA上发现的速率的50%。动力学实验表明，这种抑制不是由于干扰核苷三磷酸的添加。对延伸速率与DNA熔解温度依赖性的分析表明，染色质蛋白会干扰RNA聚合酶沿DNA模板的移位。