猿猴空泡病毒40转录复合体。I.病毒染色质蛋白对内源性RNA聚合酶活性的影响。
The sv40 transcription complex. I. Effect of viral chromatin proteins on endogenous RNA polymerase activity.
作者信息
Brooks T L, Green M H
出版信息
Nucleic Acids Res. 1977 Dec;4(12):4261-77. doi: 10.1093/nar/4.12.4261.
Abstract
SV40 chromatin obtained from infected monkey cells was used to study the effect of the viral chromatin proteins on endogenous RNA polymerase II. Ammonium sulfate activated the rate of transcription by endogenous RNA polymerase in two ways: 1) by direct action on the enzyme; and 2) by causing a reversible conformational change in the viral chromatin. Under optimal reaction conditions, the viral chromatin proteins did not limit the rate of RNA chain elongation, and high molecular weight RNA (1.6 X 10(6) d) was synthesized by the SV40 chromatin.
摘要
从受感染的猴细胞中获得的SV40染色质被用于研究病毒染色质蛋白对内源RNA聚合酶II的影响。硫酸铵通过两种方式激活内源RNA聚合酶的转录速率：1）通过对酶的直接作用；2）通过引起病毒染色质的可逆构象变化。在最佳反应条件下，病毒染色质蛋白并不限制RNA链延伸的速率，并且SV40染色质合成了高分子量RNA（1.6×10⁶ d）。
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