酵母中转录RNA基因的排列
Arangement of transfer-RNA -genes in yeast.
作者信息
Feldman H
出版信息
Nucleic Acids Res. 1976 Sep;3(9):2379-86. doi: 10.1093/nar/3.9.2379.
Abstract
The redundancy and the arrangement of the genes for specific transfer ribonucleic acids in yeast were studied by the hybridization techniques developed by Birnstiel et al., e.g.[1]. The redundancy was found to be in the order of 10 genes for tRNA1Met, tRNA3Met, tRNA2Ser, and tRNA-Pro. High molecular weight yeast DNA was fractionated by density gradient centrifugation in cesium chloride and the [32p]tRNAs were hybridized to the single fractions. The results together with earlier findings [2] suggest that the cistrons for these tRNAs are arranged in tandem interspersed by 6 to 10 times longer segments of spacer DNA which varies in (G+C) content for the different tRNA species.
摘要
利用伯恩斯蒂尔等人[1]开发的杂交技术，对酵母中特定转移核糖核酸（tRNA）的基因冗余和排列进行了研究。发现tRNA1Met、tRNA3Met、tRNA2Ser和tRNA-Pro的基因冗余度约为10个基因。通过在氯化铯中进行密度梯度离心对高分子量酵母DNA进行分级分离，并将[32P]tRNAs与单个级分进行杂交。这些结果与早期发现[2]共同表明，这些tRNA的顺反子串联排列，中间穿插着比其长6至10倍的间隔DNA片段，不同tRNA种类的间隔DNA片段的（G+C）含量有所不同。
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