Schuster D P, Kaplan J D, Gauvain K, Welch M J, Markham J
Department of Internal Medicine, Washington University Medical School, St. Louis, Missouri.
J Nucl Med. 1995 Mar;36(3):371-7.
We have previously reported a method for measuring regional pulmonary blood flow (PBF) in experimental animals using 15O-water and PET. The method requires withdrawing blood from the pulmonary artery during the PET scan, so that the input function can be estimated for the one-compartment model used to analyze the data. The purpose of the present study was to modify and validate this technique for a more general use in humans.
PBF was measured after injections of 15O-water in 15 normal subjects and in five patients with reduced cardiac output. In ten of the normal subjects, PBF was also measured after the injection of 68Ga-albumin macroaggregates (MAA). In the five other normal subjects and in the cardiomyopathy patients, PBF was measured twice after two separate 15O-water administrations. The input function was estimated from a region of interest (ROI) over the right ventricle (RV), with corrections when necessary, for time delays between RV and lung tissue.
The mean value for PBF in the normal subjects was 121 +/- 32 ml/min/100 ml lung, and was 57 +/- 33 ml/min/100 ml lung in the patients with cardiomyopathy. The correlation between PBF measured with 15O-water and PBF measured with 68Ga-MAA was r = 0.96. There was no significant difference in the mean value for PBF or the ventral-dorsal distribution of PBF when sequential measurements were made in the same individual. PBF increased in general in the ventral-dorsal direction in these supine subjects, although PBF was more evenly distributed in the cardiomyopathy patients.
Measurement of regional PBF with 15O-water and PET appears to be a valid, noninvasive approach for evaluating the pulmonary perfusion pattern of humans.
我们之前报道了一种使用(^{15}O) - 水和正电子发射断层扫描（PET）测量实验动物局部肺血流量（PBF）的方法。该方法需要在PET扫描期间从肺动脉抽血，以便为用于分析数据的单室模型估计输入函数。本研究的目的是改进并验证该技术，以便更广泛地应用于人类。
对15名正常受试者和5名心输出量降低的患者注射(^{15}O) - 水后测量PBF。在10名正常受试者中，注射(^{68}Ga) - 白蛋白大聚合体（MAA）后也测量了PBF。在另外5名正常受试者和心肌病患者中，在两次单独注射(^{15}O) - 水后分别测量了两次PBF。通过右心室（RV）上的感兴趣区域（ROI）估计输入函数，并在必要时对RV和肺组织之间的时间延迟进行校正。
正常受试者的PBF平均值为121±32 ml/min/100 ml肺，心肌病患者为57±33 ml/min/100 ml肺。用(^{15}O) - 水测量的PBF与用(^{68}Ga) - MAA测量的PBF之间的相关性为r = 0.96。在同一个体中进行连续测量时，PBF的平均值或PBF的腹 - 背部分布没有显著差异。在这些仰卧位受试者中，PBF一般在腹 - 背方向增加，尽管在心肌病患者中PBF分布更均匀。
用(^{15}O) - 水和PET测量局部PBF似乎是评估人类肺灌注模式的一种有效、非侵入性方法。
