利用金黄色葡萄球菌的125I蛋白A检测循环IgG聚集体和免疫复合物。
Detection of circulating IgG aggregates and immune complexes using 125I protein A from Staphylococcus aureus.
作者信息
Hällgren R, Wide L
出版信息
Ann Rheum Dis. 1976 Aug;35(4):306-13. doi: 10.1136/ard.35.4.306.
Abstract
A method for detection of circulating immune complexes by the use of 125I labelled staphylococcal protein A is described. In a polyethylene glycol solution as little as 1-2 mg/l of soluble heat aggregated human IgG could be quantitated. Variables which might influence the assay were examined. Separation of immune complexes in serum from monomeric IgG was essential and achieved by gel chromatography on Sephadex G200. This assay may be suitable for clinical routine for detection and quantitation of immune complexes. A preliminary study on the clinical application of the method is presented. 58% of patients with systemic lupus erythematosus and 42% of patients with rheumatoid arthritis had increased levels of immune aggregates in serum compared to a group of healthy individuals.
摘要
本文描述了一种使用¹²⁵I标记的葡萄球菌蛋白A检测循环免疫复合物的方法。在聚乙二醇溶液中，低至1 - 2mg/l的可溶性热聚集人IgG都可被定量。研究了可能影响该检测的变量。血清中免疫复合物与单体IgG的分离至关重要，通过在葡聚糖凝胶G200上进行凝胶色谱法实现。该检测方法可能适用于临床常规检测和定量免疫复合物。文中给出了该方法临床应用的初步研究。与一组健康个体相比，58%的系统性红斑狼疮患者和42%的类风湿关节炎患者血清中免疫聚集体水平升高。
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