Boss P K, Gardner R C, Janssen B J, Ross G S
Centre for Gene Technology, School of Biological Sciences, University of Auckland, New Zealand.
Plant Mol Biol. 1995 Jan;27(2):429-33. doi: 10.1007/BF00020197.
A full-length cDNA clone encoding apple (Malus domesticus) polyphenol oxidase (PPO) was isolated from a fruit peel cDNA library. Southern analysis indicated that apple PPO is encoded by a divergent multigene family. By northern analysis, PPO mRNA was only detected in a fruit sample taken one week after full bloom. PPO mRNA accumulated in wounded tissues, and also in peel tissue showing the symptoms of superficial scald, a post-harvest disorder. The induction of PPO mRNA provides the first evidence for transcriptional control of PPO expression after wounding or the manifestation of a physiological disorder.
从苹果（苹果属）果皮cDNA文库中分离出一个编码多酚氧化酶（PPO）的全长cDNA克隆。Southern分析表明，苹果PPO由一个不同的多基因家族编码。通过Northern分析，仅在盛花后一周采集的果实样品中检测到PPO mRNA。PPO mRNA在受伤组织中积累，也在表现出浅表烫伤症状（一种采后病害）的果皮组织中积累。PPO mRNA的诱导为受伤或生理病害表现后PPO表达的转录调控提供了首个证据。
