Bouzid M, Djennaoui D, Dubreuil J, Bouguermouh A, Ellouz D, Abdelwahab J, Decaussin G, Ooka T
Laboratoire de Virologie Moléculaire, IVMC, UMR30 CNRS-UCLB, Faculté de Médecine Alexis Carrel, Lyon, France.
Int J Cancer. 1994 Feb 15;56(4):468-73. doi: 10.1002/ijc.2910560403.
The genotypes of Epstein-Barr virus (EBV) were investigated in North African nasopharyngeal carcinoma (NPC) biopsies, nasopharyngeal chronic inflammation (NCI) biopsies, and saliva of healthy individuals from Algeria and Tunisia where there is an intermediate incidence of NPC. The prevalence of A-type virus in NPC, NCI biopsies and saliva of healthy individuals was found in these regions by means of a PCR assay. Restriction enzyme polymorphism analysis by Southern blotting revealed that all North African EBV variants have a conserved restriction site on BamHI W'-I' and XhoI LMP gene. No additional BamHI enzyme site on the BamHI-F fragment was observed; however, the presence of an extra BamHI site on the BamHI-H fragment giving 2 HI and H2 fragment-like EBV M-ABA strains was found. All EBV strains present in NPC or NCI biopsies at all ages were homogeneous in these polymorphisms and no correlation was observed between the EBV genotypes from NPC patients and clinical stages of the cancer. These characteristics revealed a significant difference between the EBV variants common in Chinese NPC and those in North African NPC.
在阿尔及利亚和突尼斯（鼻咽癌发病率处于中等水平），对北非鼻咽癌（NPC）活检组织、鼻咽部慢性炎症（NCI）活检组织以及健康个体的唾液样本进行了爱泼斯坦-巴尔病毒（EBV）基因型研究。通过聚合酶链反应（PCR）检测方法，发现这些地区的NPC、NCI活检组织以及健康个体唾液中存在A型病毒。通过Southern印迹法进行的限制性内切酶多态性分析显示，所有北非EBV变体在BamHI W'-I'和XhoI LMP基因上都有一个保守的限制性酶切位点。在BamHI-F片段上未观察到额外的BamHI酶切位点；然而，在BamHI-H片段上发现了一个额外的BamHI位点，产生了类似EBV M-ABA菌株的2个HI和H2片段。所有年龄段NPC或NCI活检组织中存在的EBV菌株在这些多态性方面是一致的，且未观察到NPC患者的EBV基因型与癌症临床分期之间存在相关性。这些特征揭示了中国NPC中常见的EBV变体与北非NPC中常见的EBV变体之间存在显著差异。
