Lung M L, Chang R S, Huang M L, Guo H Y, Choy D, Sham J, Tsao S Y, Cheng P, Ng M H
Department of Microbiology, Queen Mary Hospital, University of Hong Kong.
Virology. 1990 Jul;177(1):44-53. doi: 10.1016/0042-6822(90)90458-4.
We have used probes representing approximately two-thirds of the Epstein-Barr virus (EBV) genome to examine genetic polymorphism of wild-type EBV directly detected in 28 nasopharyngeal carcinoma (NPC) tissues from Southern China. In the hypervariable regions containing reiterated sequences, this analysis showed that while naturally occurring viruses are genetically diverse, only one genotype was detectable in each tumor specimen. Unique variants in NPC were detected in four regions of the EBV genome. Two of these appeared significant in their distribution. The type C variant resulting from loss of a BamHl site between the BamHl W1* and l1* regions was prevalent among isolates from Southern China, but not so common in the United States. An "f" variant having an extra BamHl site in the BamHl F region was almost exclusively detected in only Asian NPC biopsies.
我们使用了代表约三分之二爱泼斯坦-巴尔病毒（EBV）基因组的探针，来检测在中国南方28例鼻咽癌（NPC）组织中直接检测到的野生型EBV的基因多态性。在含有重复序列的高变区，该分析表明，虽然自然存在的病毒在基因上是多样的，但在每个肿瘤标本中只能检测到一种基因型。在EBV基因组的四个区域检测到鼻咽癌中的独特变异。其中两个在其分布上显得很显著。由BamHl W1和l1区域之间的BamHl位点缺失导致的C型变异在中国南方的分离株中很普遍，但在美国则不那么常见。在BamHl F区域有一个额外BamHl位点的“f”变异几乎只在亚洲鼻咽癌活检中检测到。
