Lisby G
Department of Clinical Microbiology, Herlev Hospital, Denmark.
Acta Neurol Scand. 1993 Dec;88(6):385-7. doi: 10.1111/j.1600-0404.1993.tb05364.x.
A retroviral etiology to MS has been sought for some time, so far reports of retroviral presence have not been confirmed by other groups. DNA was isolated from peripheral blood mononuclear cells from 67 patients with MS and brain capillaries from six patients with MS. Enzymatic amplification by the polymerase chain reaction was conducted with ten primer sets homologous to highly conserved HTLV-I/HTLV-II genetic sequences at stringent and non-stringent annealing conditions. No HTLV I/II related DNA fragments were seen judging from hybridization to an HTLV-I probe, even at relaxed conditions. The present study does not support a role for a HTLV-I-like virus in MS.
一段时间以来，人们一直在探寻多发性硬化症的逆转录病毒病因，然而到目前为止，其他研究小组尚未证实有关逆转录病毒存在的报告。从67例多发性硬化症患者的外周血单核细胞以及6例多发性硬化症患者的脑毛细血管中提取了DNA。在严格和非严格退火条件下，使用与高度保守的人类嗜T淋巴细胞病毒I型/人类嗜T淋巴细胞病毒II型（HTLV-I/HTLV-II）基因序列同源的10组引物进行聚合酶链反应酶促扩增。即使在宽松条件下，根据与HTLV-I探针的杂交情况，也未发现与HTLV I/II相关的DNA片段。本研究不支持类HTLV-I病毒在多发性硬化症中起作用。
