Higashiyama Y, Katamine S, Kohno S, Mukae H, Hino S, Miyamoto T, Hara K
Second Department of Internal Medicine, Nagasaki University School of Medicine, Japan.
Clin Exp Immunol. 1994 May;96(2):193-201. doi: 10.1111/j.1365-2249.1994.tb06541.x.
Accumulating evidence has suggested the involvement of HTLV-1 in the inflammatory lesions of various organs, including the lung. However, the causal relationship between HTLV-1 and inflammatory responses in the organs remains to be elucidated. In order to evaluate the expression of HTLV-1 and its effects in the lung, we examined the expression of mRNA for the HTLV-1 tax/rex gene in fresh bronchoalveolar lavage cells (BALC) and peripheral blood mononuclear cells (PBMC) of 23 seropositive individuals, including six patients with HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP), by use of an improved method of reverse transcription-polymerase chain reaction (RT-PCR). The tax/rex mRNA was more frequently detected in BALC than in PBMC. All the HAM/TSP patients and eight of 17 carriers without neurological symptoms showed the expression of tax/rex mRNA in the BALC. IgM class antibodies to HTLV-1 were preferentially detected in sera of the tax/rex mRNA-positive individuals. The detection of tax/rex mRNA correlated closely with the presence of lymphocytosis accompanied by an elevated proportion of IL-2 receptor-bearing T cells in the BALC. Our findings indicate the crucial role of viral expression in the inflammatory response in the lung in HTLV-1-infected individuals.
越来越多的证据表明，人类嗜T淋巴细胞病毒1型（HTLV-1）与包括肺在内的各种器官的炎性病变有关。然而，HTLV-1与这些器官炎症反应之间的因果关系仍有待阐明。为了评估HTLV-1在肺中的表达及其影响，我们采用改进的逆转录聚合酶链反应（RT-PCR）方法，检测了23名血清反应阳性个体（包括6例HTLV-1相关脊髓病/热带痉挛性截瘫患者）新鲜支气管肺泡灌洗细胞（BALC）和外周血单个核细胞（PBMC）中HTLV-1 tax/rex基因的mRNA表达。与PBMC相比，tax/rex mRNA在BALC中更常被检测到。所有HAM/TSP患者以及17名无神经症状携带者中的8名，其BALC中均检测到tax/rex mRNA的表达。在tax/rex mRNA阳性个体的血清中，优先检测到HTLV-1的IgM类抗体。tax/rex mRNA的检测与BALC中淋巴细胞增多以及携带IL-2受体的T细胞比例升高密切相关。我们的研究结果表明，病毒表达在HTLV-1感染个体的肺部炎症反应中起关键作用。
