Wilcox M H, Schumacher-Perdreau F
Department of Experimental and Clinical Microbiology, University of Sheffield Medical School, UK.
J Hosp Infect. 1994 Apr;26(4):239-50. doi: 10.1016/0195-6701(94)90014-0.
The relevance of adherent growth of coagulase-negative staphylococci (CNS) in vitro to their clinical significance is controversial. We have investigated the accumulation on polymer surfaces of clinically significant CNS strains, isolated from blood cultures and intravascular catheter tips, compared with controls. The adherent growth of significant CNS isolates in a microtitre tray assay was not greater than controls, and this finding was repeated when different combinations of target polymer surface, culture medium and incubation atmosphere type were investigated. The surface accumulation of CNS cultured in pooled human serum was poor compared with culture in broth. Whole cell and surface protein profiles of serum grown Staphylococcus epidermidis differed markedly from broth cultured cells. We recommend the use of human body fluids, such as serum, as useful culture media to assess whether potential virulence determinants are likely to be expressed in vivo. The microtitre tray assay of adherent growth is not helpful in determining the virulence of individual CNS isolates.
凝固酶阴性葡萄球菌（CNS）体外贴壁生长与其临床意义之间的相关性存在争议。我们研究了从血培养物和血管内导管尖端分离出的具有临床意义的CNS菌株在聚合物表面的积聚情况，并与对照组进行了比较。在微量滴定板试验中，具有临床意义的CNS分离株的贴壁生长并不比对照组强，并且在研究靶聚合物表面、培养基和培养气氛类型的不同组合时，这一发现得到了重复。与在肉汤中培养相比，在混合人血清中培养的CNS的表面积聚较差。血清培养的表皮葡萄球菌的全细胞和表面蛋白谱与肉汤培养的细胞明显不同。我们建议使用人体体液，如血清，作为有用的培养基，以评估潜在的毒力决定因素是否可能在体内表达。微量滴定板贴壁生长试验无助于确定单个CNS分离株的毒力。
