Valera C V, Shute G T
Bull World Health Organ. 1976;53(4):391-8.
Previous investigations had shown that resistance to standard regimens of chloroquine occurred in some cases of falciparum infection in the Philippines. More extensive investigations into this phenomenon were planned by the Malaria Eradication Service, by means of the in vitro technique developed by Rieckmann, in order to determine both the distribution of resistant strains throughout the country and their local incidence. Before these studies were undertaken, a series of observations were made on cases of falciparum malaria encountered in Manila and its environs, to assess the reaction of local strains of the parasite to the in vitro test. These cases were also treated with standard doses of chloroquine and some were followed up for 4 weeks to compare the predictions made as a result of the in vitro tests with the in vivo observations. Of the 34 in vitro tests carried out, 18 were followed up in vivo. In 8 cases, no recrudescence occurred, but in the other 10 recrudescences were detected during the 4-week observation period, thus indicating parasite resistance to the drug. In each of the 18 cases, the in vivo response followed the in vitro prediction.
先前的调查表明，在菲律宾，一些恶性疟原虫感染病例对氯喹标准疗法产生了抗药性。疟疾根除服务部门计划通过里希克曼开发的体外技术，对这一现象展开更广泛的调查，以确定抗药菌株在全国的分布情况及其在当地的发病率。在进行这些研究之前，对在马尼拉及其周边地区发现的恶性疟疾病例进行了一系列观察，以评估当地疟原虫菌株对体外试验的反应。这些病例也接受了标准剂量的氯喹治疗，部分病例进行了为期4周的随访，以便将体外试验结果与体内观察结果进行比较。在进行的34次体外试验中，有18次进行了体内随访。8例未出现复发，但在另外10例中，在4周观察期内检测到复发，从而表明疟原虫对该药物产生了抗药性。在这18例中的每一例中，体内反应均符合体外试验的预测。
