Shvinka N, Caffier G
Institute of Cytology, Russian Academy of Sciences, St. Petersburg.
Biophys J. 1994 Jul;67(1):143-52. doi: 10.1016/S0006-3495(94)80463-1.
Cation conductance and efflux induced by polyene antibiotics amphotericin B (AMB), amphotericin B methyl ester (AME), nystatin, mycoheptin, and levorin on frog isolated skeletal muscle fibers and whole sartorius muscles were investigated. Conductance was measured under current-clamp conditions using a double sucrose-gap technique. Cation efflux was studied using flame emission photometry. Some new data were obtained concerning the effects of levorin and mycoheptin on biological membranes. The power dependence of polyene-induced cation transport on antibiotic concentration in muscle membrane was lower than that in bilayers. The decline in the equilibrium conductance caused by polyene removal (except for levorin) was very fast. There was reverse temperature dependence of AMB- and nystatin-induced conductances. Both induced conductance and efflux values demonstrated a correlation with the order of antifungal activities: levorin > AMB, mycoheptin > AME > nystatin, except for AME, which was more potent on yeastlike cells. These effects were interpreted in terms of possible differences in the kinetics of channel formation in biological and model membranes and in light of the role of nonconducting antibiotic forms in biological membranes.
研究了多烯抗生素两性霉素B（AMB）、两性霉素B甲酯（AME）、制霉菌素、霉菌烯素和左菌素对青蛙离体骨骼肌纤维和完整缝匠肌的阳离子电导和外流的影响。使用双蔗糖间隙技术在电流钳制条件下测量电导。使用火焰发射光度法研究阳离子外流。获得了一些关于左菌素和霉菌烯素对生物膜影响的新数据。多烯诱导的阳离子转运对肌肉膜中抗生素浓度的幂依赖性低于双层膜中的情况。多烯去除（左菌素除外）导致的平衡电导下降非常快。AMB和制霉菌素诱导的电导存在反向温度依赖性。诱导的电导和外流值均与抗真菌活性顺序相关：左菌素>AMB，霉菌烯素>AME>制霉菌素，但AME对酵母样细胞更有效。根据生物膜和模型膜中通道形成动力学的可能差异以及非导电抗生素形式在生物膜中的作用对这些效应进行了解释。
