Casaril M, Corso F, Bassi A, Capra F, Gabrielli G B, Stanzial A M, Nicoli N, Corrocher R
Istituto di Patologia Medica-Cattedra di Medicina Interna, University of Verona, Italy.
Int J Clin Lab Res. 1994;24(2):94-7. doi: 10.1007/BF02593907.
To investigate the role of oxygen free radicals in hepatocellular carcinoma we assayed tissue scavenger enzymes (superoxide dismutase and selenium-dependent glutathione peroxidase) in liver homogenate, plasma concentrations of vitamins A and E and the serum selenium level from 19 control patients, 23 cases of hepatocellular carcinoma and 18 cases of metastases to liver from different carcinomas. In hepatocellular carcinoma tissue the enzyme activities were all significantly lower than in control liver and in metastases-bearing liver; the enzyme activities of the latter tissues were not different from control liver. In contrast, normal liver adjacent to the hepatocellular carcinoma had decreased activity of superoxide dismutase. Serum selenium concentrations were significantly decreased in patients with hepatocellular carcinoma and those with liver metastases, while vitamin A was significantly decreased only in the former. These findings suggest that hepatocellular carcinoma develops in liver with severe impairment of cellular antioxidant systems, since, in patients with liver metastases from different cancers, despite low selenium concentrations, cellular scavenger enzymes have normal activities.
为了研究氧自由基在肝细胞癌中的作用，我们检测了19例对照患者、23例肝细胞癌患者以及18例来自不同癌症的肝转移患者肝脏匀浆中的组织清除酶（超氧化物歧化酶和硒依赖性谷胱甘肽过氧化物酶）、血浆维生素A和E浓度以及血清硒水平。在肝细胞癌组织中，这些酶的活性均显著低于对照肝脏和有转移灶的肝脏；后两者组织的酶活性与对照肝脏无差异。相比之下，肝细胞癌旁的正常肝脏中超氧化物歧化酶活性降低。肝细胞癌患者和肝转移患者的血清硒浓度显著降低，而仅前者的维生素A显著降低。这些发现表明，肝细胞癌是在细胞抗氧化系统严重受损的肝脏中发生的，因为在来自不同癌症的肝转移患者中，尽管硒浓度较低，但细胞清除酶的活性正常。
