大肠杆菌中双链断裂引发的DNA复制：对同源重组功能的依赖性。
DNA replication triggered by double-stranded breaks in E. coli: dependence on homologous recombination functions.
作者信息
Asai T, Bates D B, Kogoma T
机构信息
Department of Cell Biology, University of New Mexico School of Medicine, Albuquerque 87131.
出版信息
Cell. 1994 Sep 23;78(6):1051-61. doi: 10.1016/0092-8674(94)90279-8.
Abstract
Homologous recombination-dependent DNA replication (RDR) of a lambda cos site-carrying plasmid is demonstrated in E. coli cells when the cells express lambda terminase that introduces a double-stranded break into the cos site. RDR occurs in normal wild-type cells if the plasmid also contains the recombination hotspot chi. Chi is dispensable when cells are induced for the SOS response or contain a recD mutation. recBC sbcA mutant cells are also capable of RDR induction. A recN mutation greatly reduces RDR in normal cells, but not in SOS-induced cells. RDR proceeds by the theta mode or rolling circle mode of DNA synthesis, yielding covalently closed circular plasmid monomers or linear plasmid multimers, respectively. Previously described inducible stable DNA replication is considered to be a special type of RDR that starts exclusively from specific sites (oriMs) on the chromosome.
摘要
当大肠杆菌细胞表达能在粘性末端位点引入双链断裂的λ末端酶时，携带λ粘性末端位点的质粒会发生同源重组依赖性DNA复制（RDR）。如果质粒还含有重组热点χ序列，RDR会在正常野生型细胞中发生。当细胞被诱导产生SOS反应或含有recD突变时，χ序列是可有可无的。recBC sbcA突变细胞也能够诱导RDR。recN突变在正常细胞中会大大降低RDR，但在SOS诱导的细胞中不会。RDR通过DNA合成的θ模式或滚环模式进行，分别产生共价闭合的环状质粒单体或线性质粒多聚体。先前描述的可诱导稳定DNA复制被认为是一种特殊类型的RDR，它仅从染色体上的特定位点（oriMs）开始。
相似文献
1
DNA replication triggered by double-stranded breaks in E. coli: dependence on homologous recombination functions.大肠杆菌中双链断裂引发的DNA复制：对同源重组功能的依赖性。
Cell. 1994 Sep 23;78(6):1051-61. doi: 10.1016/0092-8674(94)90279-8.
2
Homologous recombination-dependent initiation of DNA replication from DNA damage-inducible origins in Escherichia coli.大肠杆菌中依赖同源重组从DNA损伤诱导型起始点引发DNA复制
EMBO J. 1993 Aug;12(8):3287-95. doi: 10.1002/j.1460-2075.1993.tb05998.x.
3
Recombination of bacteriophage lambda in recD mutants of Escherichia coli.噬菌体λ在大肠杆菌recD突变体中的重组
Genome. 1989;31(1):53-67. doi: 10.1139/g89-013.
4
An Escherichia coli strain, BJ5183, that shows highly efficient conservative (two-progeny) DNA double-strand break repair of restriction breaks.一种大肠杆菌菌株BJ5183，其对限制性酶切断裂表现出高效的保守性（双子代）DNA双链断裂修复。
Gene. 2003 Jan 16;303:89-97. doi: 10.1016/s0378-1119(02)01107-1.
5
Genetic analysis of double-strand break repair in Escherichia coli.大肠杆菌双链断裂修复的遗传分析
J Bacteriol. 1993 Aug;175(16):5176-85. doi: 10.1128/jb.175.16.5176-5185.1993.
6
The recombination hot spot chi activates RecBCD recombination by converting Escherichia coli to a recD mutant phenocopy.重组热点chi通过将大肠杆菌转变为recD突变体表型模拟物来激活RecBCD重组。
Proc Natl Acad Sci U S A. 1995 Jul 3;92(14):6244-8. doi: 10.1073/pnas.92.14.6244.
7
Lethality of rep recB and rep recC double mutants of Escherichia coli.大肠杆菌rep recB和rep recC双突变体的致死率。
Mol Microbiol. 1995 Sep;17(6):1177-88. doi: 10.1111/j.1365-2958.1995.mmi_17061177.x.
8
Double-strand-break repair recombination in Escherichia coli: physical evidence for a DNA replication mechanism in vivo.大肠杆菌中的双链断裂修复重组：体内DNA复制机制的物理证据
Genes Dev. 1999 Nov 1;13(21):2889-903. doi: 10.1101/gad.13.21.2889.
9
Evidence for conservative (two-progeny) DNA double-strand break repair.保守性（双子代）DNA双链断裂修复的证据。
Genetics. 1995 Jan;139(1):5-17. doi: 10.1093/genetics/139.1.5.
10
Double-strand end repair via the RecBC pathway in Escherichia coli primes DNA replication.大肠杆菌中通过RecBC途径进行的双链末端修复引发DNA复制。
Genes Dev. 1999 Feb 1;13(3):345-56. doi: 10.1101/gad.13.3.345.
引用本文的文献
1
Prokaryotic DNA Crossroads: Holliday Junction Formation and Resolution.原核生物DNA交叉点：霍利迪连接体的形成与拆分
ACS Omega. 2024 Feb 27;9(11):12515-12538. doi: 10.1021/acsomega.3c09866. eCollection 2024 Mar 19.
2
Reactive oxygen species accumulation is synchronised with growth inhibition of temperature-sensitive recAts polA Escherichia coli.活性氧积累与温度敏感 recAts polA 大肠杆菌生长抑制同步。
Arch Microbiol. 2022 Jun 16;204(7):396. doi: 10.1007/s00203-022-02957-z.
3
Repair of DNA Breaks by Break-Induced Replication.断裂诱导复制修复 DNA 断裂。
Annu Rev Biochem. 2021 Jun 20;90:165-191. doi: 10.1146/annurev-biochem-081420-095551. Epub 2021 Apr 1.
4
Rolling-Circle Replication in Mitochondrial DNA Inheritance: Scientific Evidence and Significance from Yeast to Human Cells.线粒体DNA遗传中的滚环复制：从酵母到人类细胞的科学证据及意义
Genes (Basel). 2020 May 6;11(5):514. doi: 10.3390/genes11050514.
5
The Roles of Bacterial DNA Double-Strand Break Repair Proteins in Chromosomal DNA Replication.细菌 DNA 双链断裂修复蛋白在染色体 DNA 复制中的作用。
FEMS Microbiol Rev. 2020 May 1;44(3):351-368. doi: 10.1093/femsre/fuaa009.
6
RecBCD, SbcCD and ExoI process a substrate created by convergent replisomes to complete DNA replication.RecBCD、SbcCD 和 ExoI 可处理由两个复制叉引发的底物，以完成 DNA 复制。
Mol Microbiol. 2019 Jun;111(6):1638-1651. doi: 10.1111/mmi.14242. Epub 2019 May 6.
7
Evidence for double-strand break mediated mitochondrial DNA replication in Saccharomyces cerevisiae.酿酒酵母中双链断裂介导的线粒体DNA复制的证据。
Nucleic Acids Res. 2017 Jul 27;45(13):7760-7773. doi: 10.1093/nar/gkx443.
8
Break induced replication in eukaryotes: mechanisms, functions, and consequences.真核生物中的断裂诱导复制：机制、功能及后果
Crit Rev Biochem Mol Biol. 2017 Aug;52(4):395-413. doi: 10.1080/10409238.2017.1314444. Epub 2017 Apr 21.
9
Non-Canonical Replication Initiation: You're Fired!非经典复制起始：你被解雇了！
Genes (Basel). 2017 Jan 27;8(2):54. doi: 10.3390/genes8020054.
10
An Overview of the Molecular Mechanisms of Recombinational DNA Repair.重组DNA修复的分子机制概述
Cold Spring Harb Perspect Biol. 2015 Nov 2;7(11):a016410. doi: 10.1101/cshperspect.a016410.
本文引用的文献
1
RecQ DNA helicase of Escherichia coli. Characterization of the helix-unwinding activity with emphasis on the effect of single-stranded DNA-binding protein.大肠杆菌的RecQ DNA解旋酶。着重于单链DNA结合蛋白作用的螺旋解旋活性的表征。
J Mol Biol. 1993 Apr 20;230(4):1145-50. doi: 10.1006/jmbi.1993.1231.
2
The recombination hotspot chi is a regulatory sequence that acts by attenuating the nuclease activity of the E. coli RecBCD enzyme.重组热点chi是一种调控序列，其作用是减弱大肠杆菌RecBCD酶的核酸酶活性。
Cell. 1993 Apr 9;73(1):87-96. doi: 10.1016/0092-8674(93)90162-j.
3
Homologous recombination-dependent initiation of DNA replication from DNA damage-inducible origins in Escherichia coli.大肠杆菌中依赖同源重组从DNA损伤诱导型起始点引发DNA复制
EMBO J. 1993 Aug;12(8):3287-95. doi: 10.1002/j.1460-2075.1993.tb05998.x.
4
DNA double-strand break repair: genetic determinants of flanking crossing-over.DNA双链断裂修复：侧翼交叉互换的遗传决定因素
Proc Natl Acad Sci U S A. 1994 Feb 1;91(3):1173-7. doi: 10.1073/pnas.91.3.1173.
5
Meiosis-induced double-strand break sites determined by yeast chromatin structure.由酵母染色质结构决定的减数分裂诱导双链断裂位点
Science. 1994 Jan 28;263(5146):515-8. doi: 10.1126/science.8290959.
6
Genetic and molecular analyses of the C-terminal region of the recE gene from the Rac prophage of Escherichia coli K-12 reveal the recT gene.对来自大肠杆菌K-12的Rac原噬菌体的recE基因C末端区域进行的遗传和分子分析揭示了recT基因。
J Bacteriol. 1993 Dec;175(23):7673-82. doi: 10.1128/jb.175.23.7673-7682.1993.
7
Relating biochemistry to biology: how the recombinational repair function of RecA protein is manifested in its molecular properties.将生物化学与生物学联系起来：RecA蛋白的重组修复功能如何在其分子特性中体现。
Bioessays. 1993 Sep;15(9):617-23. doi: 10.1002/bies.950150908.
8
Homologous pairing and strand exchange promoted by the Escherichia coli RecT protein.由大肠杆菌RecT蛋白促进的同源配对和链交换。
Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):3205-9. doi: 10.1073/pnas.91.8.3205.
9
Reversible inactivation of the Escherichia coli RecBCD enzyme by the recombination hotspot chi in vitro: evidence for functional inactivation or loss of the RecD subunit.体外重组热点chi对大肠杆菌RecBCD酶的可逆失活作用：RecD亚基功能失活或缺失的证据
Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):2980-4. doi: 10.1073/pnas.91.8.2980.
10
D-loops and R-loops: alternative mechanisms for the initiation of chromosome replication in Escherichia coli.D环和R环：大肠杆菌中染色体复制起始的替代机制
J Bacteriol. 1994 Apr;176(7):1807-12. doi: 10.1128/jb.176.7.1807-1812.1994.
Zotero 插件