用于培养苛求菌的改良富集肉汤。
Improved enrichment broth for cultivation of fastidious organisms.
作者信息
Cartwright C P, Stock F, Gill V J
机构信息
Clinical Pathology Department, Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892.
出版信息
J Clin Microbiol. 1994 Jul;32(7):1825-6. doi: 10.1128/jcm.32.7.1825-1826.1994.
Abstract
An enrichment broth developed in our laboratory, fastidious broth (FB), was compared with two commercially available broth media, supplemented thioglycolate broth and enriched eugonic broth. FB supported the growth of a number of organisms that were not cultivatable in either of the other two media, including Corynebacterium jeikeium, Haemophilus influenzae, Neisseria gonorrhoeae, and Streptococcus pneumoniae. In addition, for several organisms that were able to grow in all three broths, including Neisseria meningitidis, Nocardia asteroides, and Actinomyces spp., both the time of incubation and the starting inoculum necessary to enable detection of growth were decreased significantly by using FB.
摘要
在我们实验室研发的一种增菌肉汤——苛求肉汤（FB），与两种市售肉汤培养基（硫乙醇酸盐补加肉汤和增菌嗜温肉汤）进行了比较。FB能支持许多在其他两种培养基中无法培养的微生物生长，包括杰氏棒状杆菌、流感嗜血杆菌、淋病奈瑟菌和肺炎链球菌。此外，对于能在所有三种肉汤中生长的几种微生物，包括脑膜炎奈瑟菌、星型诺卡菌和放线菌属，使用FB可显著缩短培养时间并减少能够检测到生长所需的起始接种量。
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本文引用的文献
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Laboratory diagnosis of bacterial meningitis.细菌性脑膜炎的实验室诊断
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