Yousef G E, Isenberg D A, Mowbray J F
Department of Experimental Pathology, St. Mary's Hospital Medical School, London.
Ann Rheum Dis. 1990 May;49(5):310-5. doi: 10.1136/ard.49.5.310.
A subgenomic cDNA probe with broad specificity for a range of enteroviruses was used to test by an in situ hybridisation technique for the presence of enterovirus specific genomic sequences in muscle biopsy samples obtained from patients with chronic adult myositis. Virus specific RNA sequences were detected in 6/13 (46%) patients with idiopathic polymyositis or dermatomyositis. Control samples obtained from an equal number of patients suffering from other muscle disorders were negative. A monoclonal antibody specific for an enterovirus group was used to probe for viral antigens by indirect immunoperoxidase staining; all biopsy samples from test and control groups were negative.
使用一种对多种肠道病毒具有广泛特异性的亚基因组cDNA探针，通过原位杂交技术检测从慢性成人肌炎患者获取的肌肉活检样本中肠道病毒特异性基因组序列的存在情况。在6/13（46%）的特发性多肌炎或皮肌炎患者中检测到病毒特异性RNA序列。从相同数量的患有其他肌肉疾病的患者获取的对照样本呈阴性。使用一种对肠道病毒组具有特异性的单克隆抗体，通过间接免疫过氧化物酶染色检测病毒抗原；测试组和对照组的所有活检样本均为阴性。
