• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

中国仓鼠一种新的染色体特异性着丝粒序列的克隆与分子特征分析

Cloning and molecular characterization of a novel chromosome specific centromere sequence of Chinese hamster.

作者信息

Fátyol K, Cserpán I, Praznovszky T, Keresö J, Hadlaczky G

机构信息

Institute of Genetics, Hungarian Academy of Sciences, Szeged.

出版信息

Nucleic Acids Res. 1994 Sep 11;22(18):3728-36. doi: 10.1093/nar/22.18.3728.

DOI:10.1093/nar/22.18.3728
PMID:7937084
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC308354/
Abstract

We isolated and characterized the first chromosome-specific satellite DNA (HC2sat) of Chinese hamster. This novel satellite was localized to the pericentric region of hamster chromosome 2. The 2.8 kb long repeat unit of HC2sat was identified and two such units were sequenced. Extended short range periodicity could not be revealed in repeat units. These elements are amongst the largest satellite repeat units reported from mammals to date. HC2sat is a major constituent of the pericentric region of CHO chromosome 2 representing a 7-14 Mb long DNA segment. Studies of long range organization of HC2sat indicated that the formation of the satellite array might occur in different phases and involved different amplification mechanisms.

摘要

我们分离并鉴定了中国仓鼠的首个染色体特异性卫星DNA（HC2sat）。这种新型卫星定位于仓鼠2号染色体的着丝粒周围区域。确定了HC2sat 2.8 kb长的重复单元，并对两个这样的单元进行了测序。在重复单元中未发现扩展的短程周期性。这些元件是迄今为止哺乳动物报道的最大的卫星重复单元之一。HC2sat是中国仓鼠卵巢细胞（CHO）2号染色体着丝粒周围区域的主要成分，代表一个7 - 14 Mb长的DNA片段。对HC2sat长程组织的研究表明，卫星阵列的形成可能发生在不同阶段，并涉及不同的扩增机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82ca/308354/046592776ee4/nar00042-0075-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82ca/308354/09fddb44aa05/nar00042-0071-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82ca/308354/d894df5572d7/nar00042-0073-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82ca/308354/11ff0f6bf64b/nar00042-0074-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82ca/308354/046592776ee4/nar00042-0075-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82ca/308354/09fddb44aa05/nar00042-0071-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82ca/308354/d894df5572d7/nar00042-0073-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82ca/308354/11ff0f6bf64b/nar00042-0074-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82ca/308354/046592776ee4/nar00042-0075-a.jpg

相似文献

1
Cloning and molecular characterization of a novel chromosome specific centromere sequence of Chinese hamster.中国仓鼠一种新的染色体特异性着丝粒序列的克隆与分子特征分析
Nucleic Acids Res. 1994 Sep 11;22(18):3728-36. doi: 10.1093/nar/22.18.3728.
2
Two extended arrays of a satellite DNA sequence at the centromere and at the short-arm telomere of Chinese hamster chromosome 5.中国仓鼠5号染色体着丝粒和短臂端粒处卫星DNA序列的两个延伸阵列。
Cytogenet Cell Genet. 1998;83(3-4):281-6. doi: 10.1159/000015171.
3
Sequence analysis of a functional Drosophila centromere.功能性果蝇着丝粒的序列分析。
Genome Res. 2003 Feb;13(2):182-94. doi: 10.1101/gr.681703.
4
Direct visualization of the genomic distribution and organization of two cervid centromeric satellite DNA families.两个鹿科着丝粒卫星DNA家族的基因组分布和组织的直接可视化。
Cytogenet Cell Genet. 2000;89(3-4):192-8. doi: 10.1159/000015611.
5
Structure of the pericentric long arm region of the human Y chromosome.
J Mol Biol. 1992 Nov 20;228(2):421-32. doi: 10.1016/0022-2836(92)90831-4.
6
Concerted evolution of primate alpha satellite DNA. Evidence for an ancestral sequence shared by gorilla and human X chromosome alpha satellite.灵长类α卫星DNA的协同进化。大猩猩和人类X染色体α卫星共享祖先序列的证据。
J Mol Biol. 1990 Dec 5;216(3):555-66. doi: 10.1016/0022-2836(90)90383-W.
7
Studies on the human chromosome 3 centromere with a newly cloned alphoid DNA probe.利用新克隆的α卫星DNA探针进行的人类3号染色体着丝粒研究。
Hum Hered. 1988;38(3):156-67. doi: 10.1159/000153777.
8
Isolation, cloning and characterization of two major satellite DNA families of rabbit (Oryctolagus cuniculus).兔（穴兔）两个主要卫星DNA家族的分离、克隆及特性分析
Gene. 2004 Dec 22;343(2):271-9. doi: 10.1016/j.gene.2004.09.029.
9
Gene targeting to the centromeric DNA of a human minichromosome.基因靶向人类小染色体的着丝粒DNA。
Hum Gene Ther. 1996 Jun 10;7(9):1103-9. doi: 10.1089/hum.1996.7.9-1103.
10
Molecular and cytological analysis of a 5.5 Mb minichromosome.一条5.5兆碱基对微型染色体的分子与细胞学分析
EMBO Rep. 2001 Feb;2(2):102-7. doi: 10.1093/embo-reports/kve018.

引用本文的文献

1
Tandem Repeat Diversity in Two Closely Related Hamster Species-The Chinese Hamster () and Striped Hamster ().两种近缘仓鼠物种——中国仓鼠（）和条纹仓鼠（）中的串联重复多样性
Biomedicines. 2022 Apr 18;10(4):925. doi: 10.3390/biomedicines10040925.
2
New families of site-specific repetitive DNA sequences that comprise constitutive heterochromatin of the Syrian hamster (Mesocricetus auratus, Cricetinae, Rodentia).构成叙利亚仓鼠（金仓鼠，仓鼠亚科，啮齿目）组成型异染色质的位点特异性重复DNA序列新家族。
Chromosoma. 2006 Feb;115(1):36-49. doi: 10.1007/s00412-005-0012-x. Epub 2005 Nov 23.
3
Cloning, characterization and localization of Chinese hamster HP1 isoforms.

本文引用的文献

1
Centromeric DNA cloned from functional kinetochore fragments in mitotic cells with unreplicated genomes.从具有未复制基因组的有丝分裂细胞中的功能性动粒片段克隆的着丝粒DNA。
J Cell Sci. 1993 Jun;105 ( Pt 2):359-67. doi: 10.1242/jcs.105.2.359.
2
Destabilization of tracts of simple repetitive DNA in yeast by mutations affecting DNA mismatch repair.影响DNA错配修复的突变导致酵母中简单重复DNA片段的不稳定。
Nature. 1993 Sep 16;365(6443):274-6. doi: 10.1038/365274a0.
3
Mammalian chromosome structure.哺乳动物染色体结构。
中国仓鼠HP1亚型的克隆、特性分析及定位
Chromosome Res. 2004;12(5):483-93. doi: 10.1023/B:CHRO.0000034750.34633.3c.
4
De novo chromosome formations by large-scale amplification of the centromeric region of mouse chromosomes.通过大规模扩增小鼠染色体着丝粒区域实现的从头染色体形成。
Chromosome Res. 1996 Apr;4(3):226-39. doi: 10.1007/BF02254964.
5
The structure of adenovirus type 12 DNA integration sites in the hamster cell genome.仓鼠细胞基因组中12型腺病毒DNA整合位点的结构
J Virol. 1996 Jun;70(6):3788-96. doi: 10.1128/JVI.70.6.3788-3796.1996.
Curr Opin Genet Dev. 1993 Jun;3(3):390-7. doi: 10.1016/0959-437x(93)90110-b.
4
A functional marker centromere with no detectable alpha-satellite, satellite III, or CENP-B protein: activation of a latent centromere?一个没有可检测到的α-卫星、卫星III或CENP-B蛋白的功能性标记着丝粒：一个潜在着丝粒的激活？
Am J Hum Genet. 1993 Jun;52(6):1153-63.
5
Molecular drive: a cohesive mode of species evolution.分子驱动：物种进化的一种凝聚模式。
Nature. 1982 Sep 9;299(5879):111-7. doi: 10.1038/299111a0.
6
Highly repeated sequences in mammalian genomes.哺乳动物基因组中的高度重复序列。
Int Rev Cytol. 1982;76:67-112. doi: 10.1016/s0074-7696(08)61789-1.
7
Characterization of genomic poly(dT-dG).poly(dC-dA) sequences: structure, organization, and conformation.基因组聚（dT-dG）·聚（dC-dA）序列的表征：结构、组织与构象
Mol Cell Biol. 1984 Dec;4(12):2610-21. doi: 10.1128/mcb.4.12.2610-2621.1984.
8
Bovine 1.709 satellite. Recombination hotspots and dispersed repeated sequences.牛1.709卫星。重组热点和分散重复序列。
J Mol Biol. 1984 Aug 15;177(3):399-416. doi: 10.1016/0022-2836(84)90292-4.
9
A hotspot for novel amplification joints in a mosaic of Alu-like repeats and palindromic A + T-rich DNA.在类似Alu重复序列和富含A+T的回文DNA镶嵌区域中，新型扩增接头的一个热点。
EMBO J. 1987 Aug;6(8):2401-8. doi: 10.1002/j.1460-2075.1987.tb02518.x.
10
Movement and segregation of kinetochores experimentally detached from mammalian chromosomes.从哺乳动物染色体上实验性分离的动粒的运动和分离
Nature. 1988 Nov 17;336(6196):251-4. doi: 10.1038/336251a0.