Bailey J S, Cox N A, Berrang M E
USDA, Agricultural Research Service, Russell Research Center, Athens, Georgia 30613.
Poult Sci. 1994 Jul;73(7):1153-7. doi: 10.3382/ps.0731153.
Hatchery contamination can result in exposure of newly hatched chicks to salmonellae at a time when they are most susceptible to colonization of their intestinal tract. Eggshell fragments, external rinses, and intestinal tracts from day-of-hatch chicks were sampled for salmonellae contamination. Chicks from the same hatching trays were then put in isolators or floor pens and fed a pelleted corn-soybean ration for 1 wk before external rinses and ceca from each chick were sampled for salmonellae. About 17% of eggshell, 21% of chick rinses, and 5% of intestines sampled at Day 0 were positive for salmonellae. No differences were observed between broiler hatcheries, but significant differences were seen between replications within hatching cabinets. Results from this study suggest a correlation between hatchery-acquired salmonellae and the production of potential seeder birds. No differences between eggshell and chick rinse samples were found (correlation = .81); therefore, eggshells are recommended as the best sample to determine hatchery salmonellae contamination.
孵化场污染可能导致新孵化的雏鸡在其肠道最易被定植的时候接触沙门氏菌。对孵化当天雏鸡的蛋壳碎片、外部冲洗液和肠道进行采样，检测沙门氏菌污染情况。然后将来自同一孵化盘的雏鸡放入隔离器或地面围栏中，饲喂颗粒状玉米 - 大豆日粮1周，之后对每只雏鸡的外部冲洗液和盲肠进行采样，检测沙门氏菌。在第0天采样的蛋壳中约17%、雏鸡冲洗液中约21%以及肠道中约5%的样本沙门氏菌呈阳性。肉鸡孵化场之间未观察到差异，但孵化箱内的重复试验之间存在显著差异。本研究结果表明，孵化场获得的沙门氏菌与潜在带菌鸡的产生之间存在相关性。在蛋壳和雏鸡冲洗液样本之间未发现差异（相关性 = 0.81）；因此，建议将蛋壳作为确定孵化场沙门氏菌污染的最佳样本。
