Nakamura N, Azuma C, Akamizu T, Sugawa H, Matsuda F, Mitsuda N, Honjo T, Mori T, Yamaji K
Department of Nursing, School of Allied Health Sciences, Faculty of Medicine, Osaka University, Japan.
Biochem Biophys Res Commun. 1994 Sep 30;203(3):1789-94. doi: 10.1006/bbrc.1994.2394.
From one patient with systemic lupus erythematosus retaining lupus anticoagulant (LAC), we established 6 Epstein-Barr virus-transformed human B cell clones secreting antibodies that affect the coagulation assay. Two and 4 of the clones secreted IgM and IgG antibodies, respectively. Although all 6 antibodies displayed anticardiolipin activity in ELISA, the increased binding activity in the presence of beta 2-glycoprotein I was limited only to the IgG antibodies. Five antibodies (two IgM and three IgG) had LAC activity which prolonged the activated partial thromboplastin time (APTT), whereas one IgG antibody shortened the APTT. Two of the IgG producing clones had an identical Ig heavy chain gene rearrangement despite their opposite effects on the coagulation assay. These results demonstrated the heterogeneity of LACs and diversity among their physiological functions.
从一名患有系统性红斑狼疮且保留狼疮抗凝物（LAC）的患者身上，我们建立了6个爱泼斯坦-巴尔病毒转化的人B细胞克隆，这些克隆分泌影响凝血检测的抗体。其中2个克隆分泌IgM抗体，4个克隆分泌IgG抗体。虽然所有6种抗体在ELISA中均显示出抗心磷脂活性，但仅IgG抗体在β2-糖蛋白I存在时结合活性增加。5种抗体（2种IgM和3种IgG）具有LAC活性，可延长活化部分凝血活酶时间（APTT），而1种IgG抗体则缩短了APTT。尽管对凝血检测有相反作用，但2个产生IgG的克隆具有相同的Ig重链基因重排。这些结果证明了LAC的异质性及其生理功能的多样性。
