Agullo G, Gamet L, Besson C, Demigné C, Rémésy C
Laboratoire des Maladies Métaboliques, INRA Theix, Ceyrat, France.
Cancer Lett. 1994 Nov 25;87(1):55-63. doi: 10.1016/0304-3835(94)90409-x.
The effect of the naturally occurring flavonol, quercetin, was investigated on cell growth and metabolism of two human carcinoma cell lines, HT29 and Caco-2 cells, both during the exponentially growing phase and after confluence. Our results show clearly that, after a 48-h period of treatment, quercetin (in the range of concentration from 15 microM to 120 microM) exerted a preferential cytotoxic effect on active proliferating cells. This effect was dose dependent and was accompanied by a simultaneous inhibition of lactate release and a dramatic decrease of total cellular ATP content. In contrast, in confluent cells, quercetin failed to affect cell viability or lactate release, but led nevertheless to a depletion of cellular ATP level. In conclusion, the cytotoxicity of quercetin is markedly higher in actively growing cells in comparison with confluent cells.
研究了天然存在的黄酮醇槲皮素对两种人癌细胞系HT29和Caco-2细胞在指数生长期及汇合后的细胞生长和代谢的影响。我们的结果清楚地表明，在处理48小时后，槲皮素（浓度范围为15微摩尔至120微摩尔）对活跃增殖的细胞具有优先的细胞毒性作用。这种作用呈剂量依赖性，同时伴随着乳酸释放的抑制和细胞总ATP含量的显著降低。相比之下，在汇合细胞中，槲皮素未能影响细胞活力或乳酸释放，但却导致细胞ATP水平的消耗。总之，与汇合细胞相比，槲皮素在活跃生长的细胞中的细胞毒性明显更高。
