Zha H, Wilder R L, Goldmuntz E A, Cash J M, Crofford L J, Mathern P, Remmers E F
Arthritis and Rheumatism Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892.
Cytogenet Cell Genet. 1993;63(2):117-23. doi: 10.1159/000133514.
Analysis of F2 intercross progeny of inbred F344/N x LEW/N rats led to the assignment of 10 polymorphic PCR-typable markers to rat chromosome 2. The markers form a single linkage group covering 47.9 cM with the following order: D2N1R-D2N28-FGG (gamma fibrinogen)-PKLR (liver and RBC pyruvate kinase)-ATP1A1 (the alpha-1 polypeptide of Na+/K+ transporting ATPase)-HSD3B (hydroxy-delta-5-steroid dehydrogenase)-D2N2R-D2N91-CAMKI (calmodulin-dependent protein kinase II)-D2N35. All but two of the markers (D2N1R and D2N2R) were detected using specific PCR primers flanking dinucleotide repeats. Sequences with dinucleotide repeats associated with five genes (FGG, PKLR, ATP1A1, HSD3B, and CAMKI) were identified in GenBank, and primers were designed to flank these repeats. The PCR primer pairs for three anonymous markers (D2N28, D2N91, and D2N35) were identified by sequencing cloned LEW/N rat genomic DNA containing (CA)n.(GT)n repeats. D2N1R and D2N2R were identified by PCR amplification of genomic DNA with single, nonspecific 10-base oligonucleotide primers. All of the markers were codominant except for D2N1R, D2N2R, and CAMKI, which only amplified from F344/N homozygous and heterozygous rat DNA. The seven codominant markers were highly polymorphic in 10 other inbred rat strains (SHR/N, WKY/N, MNR/N, MR/N, LOU/MN, BN/SsN, BUF/N, WBB1/N, WBB2/N, and ACI/N), suggesting that they will be useful for general mapping studies among these strains. Comparative gene mapping analysis indicated that a portion of the mapped region of rat chromosome 2 exhibits synteny conservation with regions of human chromosome 1 and mouse Chromosome 3.
对近交系F344/N与LEW/N大鼠的F2杂交后代进行分析，结果将10个多态性PCR可分型标记定位于大鼠第2号染色体上。这些标记形成一个单一的连锁群，覆盖47.9厘摩，顺序如下：D2N1R - D2N28 - FGG（γ纤维蛋白原） - PKLR（肝脏和红细胞丙酮酸激酶） - ATP1A1（Na+/K+转运ATP酶的α-1多肽） - HSD3B（羟基-δ-5-类固醇脱氢酶） - D2N2R - D2N91 - CAMKI（钙调蛋白依赖性蛋白激酶II） - D2N35。除了两个标记（D2N1R和D2N2R）外，其余所有标记均使用位于二核苷酸重复序列两侧的特异性PCR引物进行检测。在GenBank中鉴定出与五个基因（FGG、PKLR、ATP1A1、HSD3B和CAMKI）相关的二核苷酸重复序列，并设计引物位于这些重复序列两侧。通过对含有(CA)n.(GT)n重复序列的克隆LEW/N大鼠基因组DNA进行测序，鉴定出三个匿名标记（D2N28、D2N91和D2N35）的PCR引物对。D2N1R和D2N2R通过用单一的非特异性10碱基寡核苷酸引物对基因组DNA进行PCR扩增来鉴定。除D2N1R、D2N2R和CAMKI外，所有标记均为共显性，后三者仅能从F344/N纯合和杂合大鼠DNA中扩增出来。这七个共显性标记在其他10个近交大鼠品系（SHR/N、WKY/N、MNR/N、MR/N、LOU/MN、BN/SsN、BUF/N、WBB1/N、WBB2/N和ACI/N）中具有高度多态性，表明它们将有助于这些品系间的一般图谱研究。比较基因图谱分析表明，大鼠第2号染色体上已定位区域的一部分与人第1号染色体和小鼠第3号染色体区域表现出同源性保守。
