Rotter B A, Thompson B K, Lessard M, Trenholm H L, Tryphonas H
Centre for Food and Animal Research, Agriculture and Agri-Food Canada, Ottawa, Ontario.
Fundam Appl Toxicol. 1994 Jul;23(1):117-24. doi: 10.1006/faat.1994.1087.
The effects of low dietary concentrations of Fusarium mycotoxins (deoxynivalenol (DON), 15-acetyl-DON, and zearalenone) on growth, immunological, and hematological parameters were determined in young pigs during a 28-day feeding experiment. Clean and naturally contaminated corn were incorporated into basal diets formulated to contain 0.00, 0.75, 1.50, and 3.00 mg DON/kg diet. A pair-fed control animal was used for comparison with each animal receiving the highest level of contamination (diet 4). Skin temperature, measured during the first week of the experiment, decreased linearly as the dietary mycotoxin concentration increased. Several other linear effects were observed: depressed feed intake throughout the experiment, reduction in thyroid size (absolute/relative), and changes in the appearance of the esophageal region of the stomach (thicker and higher degree of folding with increasing toxin concentration). Serum T4 (thyroxine) levels increased quadratically after 7 and 28 days of exposure compared to control animals. This change coincided with an increase in albumin levels, a decrease in alpha-globulin levels, and an overall increase in albumin/globulin ratio as the level of contamination increased. After immunization with sheep red blood cells (SRBC), animals fed contaminated diets showed a delayed response in peak titers. At the end of the experiment an increase in the segmented neutrophil count was observed. The following observations were made for animals consuming diet 4 as compared to the pair-fed controls: lower skin temperature, better feed efficiency, more corrugated stomachs, reduced alpha-globulin levels, and lower antibody titers to SRBC.(ABSTRACT TRUNCATED AT 250 WORDS)
在一项为期28天的饲养实验中，测定了低日粮浓度的镰刀菌霉菌毒素（脱氧雪腐镰刀菌烯醇（DON）、15 - 乙酰基 - DON和玉米赤霉烯酮）对幼猪生长、免疫和血液学参数的影响。将清洁的和天然受污染的玉米添加到基础日粮中，基础日粮配制为分别含有0.00、0.75、1.50和3.00毫克DON/千克日粮。使用配对饲喂的对照动物与接受最高污染水平（日粮4）的每只动物进行比较。在实验的第一周测量的皮肤温度随着日粮霉菌毒素浓度的增加呈线性下降。还观察到其他一些线性效应：整个实验期间采食量降低、甲状腺大小（绝对/相对）减小以及胃食管区域外观变化（随着毒素浓度增加，褶皱更厚且程度更高）。与对照动物相比，暴露7天和28天后血清T4（甲状腺素）水平呈二次方增加。这种变化与白蛋白水平升高、α - 球蛋白水平降低以及随着污染水平增加白蛋白/球蛋白比值总体增加相一致。用绵羊红细胞（SRBC）免疫后，饲喂受污染日粮的动物在峰值滴度方面反应延迟。在实验结束时，观察到分叶中性粒细胞计数增加。与配对饲喂的对照相比，对食用日粮4的动物有以下观察结果：皮肤温度较低、饲料效率更高、胃褶皱更多、α - 球蛋白水平降低以及对SRBC的抗体滴度较低。（摘要截断于250字）
