Jones P J
Division of Human Nutrition, University of British Columbia, Vancouver, Canada.
Lipids. 1994 Jul;29(7):491-5. doi: 10.1007/BF02578246.
The objective of this study was to examine whether whole body oxidation rates of dietary linoleic, alpha-linolenic and oleic acids differ when the acids are provided in identical quantities. Male rats were fed for 10 wk a 15% fat (w/w) diet containing equal amounts of linoleic, alpha-linolenic and oleic acids (22.7, 23.0 and 23.2% of total fatty acids, respectively). At week 10, after overnight fasting, rats were intragastrically administered 20 microCi of either [1-14C]-labelled linoleic, alpha-linolenic or oleic acid in a 200-microL bolus of oil containing equal quantities of each fatty acid. The appearance of 14CO2 in expired air was then monitored hourly for 12 h for each animal. A preliminary study had shown that growth and food consumption patterns in animals consuming the oil containing equal quantities of each of the fatty acids paralleled the patterns of animals that were self-selecting among separate diets, each of which contained one of the component oils. The appearance of 14C, expressed as percent dose administered, peaked at 2-3 h post-dose for 14C-labelled linoleic (5.28 +/- 0.37%/h), alpha-linolenic (6.92 +/- 0.51%/h) and oleic (5.98 +/- 0.44%/h) acids. Statistically these values were not significantly different. Cumulative 14CO2 excretion rates over 12 h were also similar for linoleic (27.2 +/- 0.9%), alpha-linolenic (26.8 +/- 1.2%) and oleic (25.9 +/- 1.2%) acids. The results suggest that the rat's capacity to oxidize 18-carbon unsaturated fatty acids is not affected by fatty acid unsaturation when these fatty acids are provided at equal dietary levels.
本研究的目的是检验当膳食中亚油酸、α-亚麻酸和油酸以相同数量提供时，它们的全身氧化率是否存在差异。雄性大鼠被喂食一种含15%脂肪（w/w）的饮食，该饮食含有等量的亚油酸、α-亚麻酸和油酸（分别占总脂肪酸的22.7%、23.0%和23.2%），持续10周。在第10周，大鼠过夜禁食后，通过胃内给予20微居里的[1-14C]标记的亚油酸、α-亚麻酸或油酸，溶于200微升含有等量每种脂肪酸的油团中。然后每只动物每小时监测呼出气体中14CO2的出现情况，持续12小时。一项初步研究表明，食用含有等量每种脂肪酸的油的动物的生长和食物消耗模式与在单独饮食中自行选择的动物模式相似，每种单独饮食都含有一种成分油。以给药剂量的百分比表示的14C的出现情况，在给药后2 - 3小时达到峰值，14C标记的亚油酸为（5.28±0.37%/小时），α-亚麻酸为（6.92±0.51%/小时），油酸为（5.98±0.44%/小时）。从统计学上看，这些值没有显著差异。亚油酸（27.2±0.9%）、α-亚麻酸（26.8±1.2%）和油酸（25.9±1.2%）在12小时内的累积14CO2排泄率也相似。结果表明，当这些脂肪酸以相等的膳食水平提供时，大鼠氧化18碳不饱和脂肪酸的能力不受脂肪酸不饱和度的影响。
