Taylor C E, Rosenthal R O, Brown D F, Lapage S P, Hill L R, Legros R M
Equine Vet J. 1978 Jul;10(3):136-44. doi: 10.1111/j.2042-3306.1978.tb02242.x.
The aetiological agent of contagious equine metritis (CEM) has been investigated bacteriologically in a wide range of cultural and conventional biochemical tests, in the eletron microscope, for DNA base composition (36.1 per cent GC), for susceptibility to various antimicrobial agents and antigenically by means of tube and slide agglutination tests. The organism is a fastidious, Gramnegative, non acid-fast coccobacillus which in biochemical tests is very unreactive. In conventional tests, only the oxidase, catalase and phosphatase tests were positive. Dependance on neither X nor V factors could be demonstrated, but some stimulation of growth by X factor was observed. The organism could not be identified with any known species and even allocation to an appropriate characters, we propose the organism as a new species of the genus Haemophilus: H. equigenitalis, type strain NCTC 11184 (61717/77).
通过广泛的培养和传统生化试验、电子显微镜检查、DNA碱基组成分析(GC含量为36.1%)、对各种抗菌剂的敏感性测试以及试管凝集试验和玻片凝集试验等抗原性检测手段,对马传染性子宫炎(CEM)的病原体进行了细菌学研究。该病原体是一种苛求菌,革兰氏阴性,非抗酸的球杆菌,在生化试验中反应性很低。在传统试验中,只有氧化酶、过氧化氢酶和磷酸酶试验呈阳性。未发现其对X因子或V因子有依赖性,但观察到X因子对其生长有一定刺激作用。该病原体无法与任何已知菌种相鉴别,即便根据其适当特征进行归类,我们仍提议将该病原体定为嗜血杆菌属的一个新种:马生殖嗜血杆菌,模式菌株为NCTC 11184(61717/77)。