Chen S L, Lin Y K, Li L Y, Tsao Y P, Lo H Y, Wang W B, Tsai T C
Department of Microbiology & Immunology, National Defense Medical Center, Taipei, Taiwan, Republic of China.
J Virol. 1996 Dec;70(12):8558-63. doi: 10.1128/JVI.70.12.8558-8563.1996.
Human papillomavirus type 11 (HPV-11) and HPV-16 contain an E5 gene that can induce c-fos gene expression in mouse fibroblasts. This study investigated the human c-fos promoter characteristics by mapping the c-fos promoter sequence with several deletion and point mutants that confer responsiveness to E5 of HPV-11 or HPV-16. The mutant studies show that NF1 binding sequences within the c-fos promoter were crucial for the induction of the c-fos gene by E5, and the gel shift assay study suggested that E5 of both HPV-11 and HPV-16 is associated, perhaps indirectly, with this NF1 element in the transactivation of the human c-fos promoter. Using an inducible system, we demonstrate that increased induction of the HPV-11 E5 gene in cells led to increased transactivation of the NF1 element. In addition, the transactivating activity of a series of HPV-11 E5 mutants on the NF1 element had a strong correlation with their respective transforming activities.
11型人乳头瘤病毒（HPV-11）和HPV-16含有一种E5基因，该基因可在小鼠成纤维细胞中诱导c-fos基因表达。本研究通过绘制c-fos启动子序列与几种缺失和点突变体，研究了人c-fos启动子的特性，这些突变体赋予了对HPV-11或HPV-16的E5的反应性。突变体研究表明，c-fos启动子内的NF1结合序列对于E5诱导c-fos基因至关重要，凝胶迁移试验研究表明，HPV-11和HPV-16的E5在人c-fos启动子的反式激活中与该NF1元件相关，可能是间接相关。使用诱导系统，我们证明细胞中HPV-11 E5基因诱导的增加导致NF1元件的反式激活增加。此外，一系列HPV-11 E5突变体对NF1元件的反式激活活性与其各自的转化活性密切相关。
